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Oligonucleotide Synthesis Service

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High-quality oligonucleotides are the foundation of modern genetic research, molecular diagnostics, and nucleic acid therapeutics. As an industry-leading biotechnology company, Creative Biogene provides comprehensive oligonucleotide synthesis services, ranging from standard DNA/RNA oligos to highly modified, application-specific sequences. With advanced phosphoramidite chemistry, automated synthesis platforms, and strict quality control systems, we deliver products with exceptional purity, accuracy, and reproducibility—suitable for both research and GMP-grade applications.

Creative Biogene's Oligonucleotide Synthesis Services

We offer comprehensive oligonucleotide synthesis services that form a fully integrated, matrix-based platform designed to support diverse research and industrial applications. Our service matrix spans standard DNA/RNA oligos, modified and functionalized oligonucleotides, long and complex sequences, high-throughput production, and GMP-compliant manufacturing.

1. Standard and Specialty Oligonucleotide Synthesis

2. Modified and Functional Oligonucleotide Synthesis

3. Application-Oriented Oligonucleotide Synthesis

Technical Capabilities

01

Advanced Purification Options

Includes all major transporters recommended by ICH, with options for customized target development.

02

High-Throughput and Scalable Production

Parallel synthesis platforms allow large-scale manufacturing with production capacity reaching tens of thousands of OD per batch, and kg-scale synthesis is available for oligonucleotide drug intermediates.

03

Extensive Modification Portfolio

Over 200 chemical and fluorescent modifications are available, including terminal groups (NH₂, SH, PO₄, DBCO, Azide), fluorophores (FAM, HEX, TAMRA, Cy3, Cy5, BHQ1/2/3), stability-enhancing modifications (LNA, 2'-OMe, 2'-MOE, PS, PNA), and affinity tags (Biotin, HRP, Peptide, Streptavidin).

04

Precision and Consistency

Automated synthesis monitoring ensures that each coupling step achieves ≥99% efficiency, with in-process control of deprotection and oxidation steps to maintain exceptional batch-to-batch consistency.

05

Comprehensive Quality Control

Every oligonucleotide is validated by HPLC, LC-MS, CE, and UV spectroscopy to verify length, modification efficiency, and integrity, and each order is accompanied by a detailed Certificate of Analysis (CoA) and QC report.

Modification and Labeling Options

CategoryExamples
Common Modifications5'/3' Phosphorylation, Thiolation, Amination, DBCO, Alkyne, Azide
Backbone/StructuralPhosphorothioate (PS), LNA, 2'-O-Me, 2'-MOE, PNA
Fluorescent/Quencher LabelsFAM, HEX, TAMRA, Cy3/Cy5, BHQ1/2/3, Dabcyl
Affinity TagsBiotin, Desthiobiotin, HRP, Streptavidin, Peptide conjugation

Client Case Studies & Research Outcomes

Case Study 1

In a study investigating T cell gene therapy for CTLA4 insufficiency, small double-stranded oligonucleotides (dsODN) were obtained from Creative Biogene. Healthy CD4+ T cells were isolated, activated, and cultured, and gene editing was performed using a gRNA in combination with the dsODN. Seventy-two hours post-editing, genomic DNA was extracted and sent to Creative Biogene for amplification, next-generation sequencing, and analysis.

The figure illustrates CRISPR/Cas9 targeting of the CTLA4 locus, HDR donor design, and resulting editing efficiency and CTLA4 expression in edited versus unedited cells.Figure 1. The figure illustrates CRISPR/Cas9 targeting of the CTLA4 locus, HDR donor design, and resulting editing efficiency and CTLA4 expression in edited versus unedited cells. (Fox TA, et al., 2022)

Why Choose Creative Biogene?

  • Global leader in oligonucleotide manufacturing
  • ISO/GMP-certified production and QC systems
  • 200+ chemical modifications available
  • Scalable synthesis from nmol to kg quantities
  • Full support from research-grade to therapeutic applications

Contact Us

Creative Biogene is committed to delivering reliable, customizable, and scalable nucleic acid synthesis solutions to global researchers and biopharmaceutical innovators. For professional consultation or GMP-grade oligonucleotide production, please contact our technical support team.

* For research use only. Not intended for any clinical use.
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