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Plant Genetic Modification Service

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Overview

Creative Biogene's Plant Genetic Transformation Platform leverages our innovative technology services platform, integrating end-to-end capabilities including gene editing, vector construction, transformation and regeneration, molecular characterization, and functional validation. We provide comprehensive services for plant research, molecular breeding, and industrial applications, covering the full workflow from gene/vector design to delivery of transgenic or edited materials.

Through advanced approaches such as multi-gene knockouts, base editing, prime editing, and targeted gene modifications, our platform supports C3/C4 model plants, major cereal crops, horticultural and economic crops, and oil crops, establishing a robust and versatile plant transformation system. We provide both modular services for research groups and deeply customized solutions, including project design, technical assessment, implementation, training, and follow-up data support.

Technology System

Gene Editing System

Gene Editing System

At Creative Biogene, precision meets innovation. Our Gene Editing System harnesses CRISPR/Cas technologies, NHEJ-mediated mutations, and template-driven homologous recombination to deliver site-specific insertions and trait-optimized modifications.

Genetic Transformation System

Genetic Transformation System

Whether performing rapid transient assays or generating stable transgenic plants, this system bridges the gap between molecular constructs and real-world phenotypes, enabling efficient organ-specific transformation and seamless regeneration of fully developed plants, accelerating research and trait development.

High-throughput Genotyping System

High-throughput Genotyping System

Speed and accuracy converge in our High-throughput Genotyping System, designed for large-scale, precise genetic analysis. Creative Biogene provides rapid assessment of editing outcomes, allelic diversity, and off-target events.

Target Species

Service Modules

Our platform is organized into five key modules, which can be used individually or combined into a complete workflow. Modules are flexible and customizable based on client needs.

Platform Advantages

  • Broad species coverage & breakthrough ability: Model plants, cereals, horticultural crops, medicinal and oil crops, including establishment of new transformation systems
  • Advanced editing technologies: Base editing (CBE/ABE), prime editing, multi-gene constructs
  • Professional QC & data transparency: Standardized reports, traceable data at every step
  • Flexible customization & cost optimization: Modular service selection to maximize efficiency and value
  • Technical support & collaborative services: Project planning, training, breeding recommendations

Applications

  • Gene function research: Knockout, editing, and overexpression for developmental, stress, and metabolic studies
  • Molecular breeding & crop improvement: Rapid generation of plants with desirable traits
  • Synthetic biology & metabolic engineering: Build new pathways, enhance metabolite production
  • Molecular breeding material creation: Deliver transgenic or edited germplasm for industrial breeding
  • Spatial gene expression studies: RNA FISH for tissue/cell-level gene expression insights

Case Study

Case Study 1

A study investigating the regulatory roles of intronic enhancers in Arabidopsis thaliana utilized Creative Biogene's pGreenII vector system to perform transient expression. Genome-wide prediction identified over 900 candidate intronic enhancers in seedlings and more than 1,200 in floral tissues. Functional validation was conducted using reporter constructs (pGreenII 0800-LUC) and effector vectors (pGreenII 62-SK) in Nicotiana benthamiana, demonstrating enhancer activity in 15 of 21 candidates. Further CRISPR/Cas-mediated deletions of three enhancers caused partial transcriptional repression and distinct developmental phenotypes.

Functional validation of SPL9 and EDT1 binding motifs in DHS7#1 and DHS7#2 enhancers through Y1H and luciferase assays.Figure 1. Functional validation of SPL9 and EDT1 binding motifs in DHS7#1 and DHS7#2 enhancers through Y1H and luciferase assays. (Meng F, et al., 2021)

Case Study 2

A stable Oryza sativa mutant line (CCRM-2208) targeting OsTGA5/rTGA2.3 (Os01g0279900) was generated by Creative Biogene using CRISPR/Cas9-mediated genome editing. The binary vector pCB-35S-Cas9-OsU6-sgRNA-HygR was constructed and introduced into Agrobacterium tumefaciens strain EHA105 for transformation of embryogenic calli derived from the Japonica cultivar Nipponbare. The tga5 loss-of-function mutation was confirmed through next-generation sequencing of a 2.2 kb genomic region encompassing the sgRNA target site, with validation performed using Oxford Nanopore sequencing and custom annotation. After propagation, mutant and wild-type seeds were preserved under controlled temperature and humidity conditions for subsequent physiological and developmental studies.

OsTGA5 affects trait canalization.Figure 2. OsTGA5 affects trait canalization. (Dunivant TS, et al., 2025)

Contact Us

Learn more about vector construction, plant transformation, molecular characterization, functional analysis, or RNA FISH services. Our technical team provides free project evaluation, technical recommendations, and preliminary quotes. Let's advance plant genome editing together.

References:

  1. Meng F, Zhao H, Zhu B, et al. Genomic editing of intronic enhancers unveils their role in fine-tuning tissue-specific gene expression in Arabidopsis thaliana. Plant Cell. 2021 Jul 19;33(6):1997-2014.
  2. Dunivant TS, Ćalić I, Gilligan C, et al. Environment-dependent selection impacts heritable developmental stability and trait canalization in rice. bioRxiv [Preprint]. 2025 Jun 16:2025.06.13.659410.
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