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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-MEL-2 is a human malignant melanoma cell line established in 1968, derived from the skin of a 60-year-old Caucasian male with metastatic melanoma. Unlike many melanoma cell lines that harbor BRAF mutations, SK-MEL-2 is characterized by a mutation in its NRAS gene (specifically located at codon 61), making it a preferred model for studying NRAS-driven melanoma. These cells exhibit a polygonal or spindle-shaped morphology and grow as an adherent monolayer. In the field of cutaneous oncology, it serves as a crucial research tool, frequently utilized to investigate the molecular signaling mechanisms of non-BRAF-mutated cutaneous melanoma, to study mechanisms of resistance to MAP kinase inhibitors, and to screen for novel targeted therapies and immunotherapeutic agents. |
| Tissue | Skin; derived from metastatic site (Thigh) |
| Disease | Malignant Melanoma |
| Morphology | Polygonal; Spindle-shaped |
| Gender | Male |
| Age | 60 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. NRAS-mutant melanoma pathogenesis and progression research 2. Study of MAPK/ERK and PI3K/Akt signaling pathways 3. Drug screening for BRAF-wildtype metastatic melanoma 4. Investigation of tumor immunology and cell surface antigen expression 5. Development of human tumor xenograft models in immunocompromised mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Karyotype | Aneuploid; modal number = 70; range = 62 to 73 |
| Genetic Profile | NRAS mutation (Q61R); BRAF wild-type; TP53 mutation (G245S); CDKN2A (p16) homozygous deletion |
| Expression Markers | Positive for S-100 and tyrosinase; maintains melanocyte-specific lineage markers |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00834 | Luciferase Reporter Cell Line - SK-MEL-2 | Inquiry |
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