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Creative Biogene offers diverse dsRNA controls as reliable positive controls for precise dsRNA assays. These controls empower researchers to induce RNA interference, study gene functions, and facilitate gene silencing studies. With a commitment to cutting-edge biotechnology solutions, Creative Biogene is at the forefront, supporting breakthroughs in genetic research and molecular biology.
Key Features of Our dsRNA Control
Double-stranded RNA (dsRNA) plays a crucial role in diverse biological processes, encompassing innate immune responses and RNA interference. Its detection is of paramount importance in research, clinical, and therapeutic contexts. Notably, discerning plant and animal viruses with dsRNA genomes assists in differentiating between bacterial and viral pathogens. To precisely detect dsRNA in vitro or in situ, we offer three selectable versions:
Case Study 1
Researchers have developed a streamlined method to mitigate the formation of immunogenic double-stranded RNA (dsRNA) byproducts during in vitro transcription (IVT) of messenger RNA (mRNA). The undesired dsRNA, a byproduct of IVT, poses challenges in mRNA production due to its similarity in size and intrinsic characteristics. The method incorporates selected chaotropic agents at optimized concentrations during IVT to create a mild denaturing environment, preventing the undesired base-pairing that leads to RNA-templated dsRNA formation. This approach significantly reduces dsRNA levels, minimizes immuno-stimulation, and enhances protein expression compared to traditional IVT. Importantly, the method may eliminate the need for costly post-IVT chromatography purifications, presenting a scalable, cost-effective, and efficient approach for mRNA manufacturing without requiring specialized reagents or altered reaction conditions.
Figure 1. Researchers assessed mRNA integrity, dsRNA content, protein expression, and immuno-stimulatory effect for mRNA variants, including dsRNA-positive control Poly(I:C). (Piao X, et al., 2022)
Case Study 2
Cytosolic innate immune sensors, such as RIG-I-like receptors, detect double-stranded RNA (dsRNA) derived from viruses and cells. Researchers discovered that Trp53 mutant mouse embryonic fibroblasts harbor endogenous dsRNA of mitochondrial origin, triggering an immune response via RIG-I-like receptors. This dsRNA induces the expression of type I interferon and proinflammatory cytokine genes. The immune-stimulatory dsRNA is cleaved by RNase L, intensifying RIG-I-like receptor activation. Interruption of mitochondrial transcription reduces the presence of this dsRNA. The study illuminates a versatile role of p53 in innate immunity and unveils complexities in the interaction between endogenous RNA and aberrant immune responses, providing insights into diseases with dysregulated immune reactions.
Figure 2. Researchers found that knockdown of RNase L in Trp53 MEFs resulted in significantly lower induction of ISG mRNA transcripts, Ifit3, Ifit1, and Irf7, compared to cells transfected with dsRNA from Trp53 MEFs. The reduced induction also extended to mRNA transcripts encoding IFN-α, Ifih1, and Ddx58. This emphasizes the crucial role of RNase L in the immune response triggered by dsRNA. Notably, the use of dsRNA-positive control further highlighted the dependence on RNase L for effective immune response induction. (Wiatrek DM, et al., 2019)
Q: How does the dsRNA Control contribute to the study of gene functions?
A: The dsRNA Control contributes to the study of gene functions by allowing for accurate and reproducible dsRNA assays. They offer researchers a reliable way to induce RNA interference and conduct gene silencing studies, facilitating understanding and discovery in genetic research and molecular biology.
Q: What detection methods does the dsRNA Control support?
A: The product supports several advanced detection methods, including immunofluorescence, ELISA, and immunoblot. This ensures robust, high-quality visualization and analysis of dsRNA in different experimental settings.
Q: What are the different versions of the dsRNA Control available?
A: Creative Biogene offers three selectable versions of dsRNA Control: The General Version, which ensures reliability in dsRNA assays, the N1-methylpseudo-UTP Version, used for studying dsRNA under modified nucleotide conditions, and the Pseudo-UTP Version, used for investigating the impact of Pseudo-UTP modifications on dsRNA functionality.
| Cat.No. | Product Name | Price |
|---|---|---|
| PDRN-001 | dsRNA control | Inquiry |
| PDRN-002 | dsRNA control (N1-methylpseudo-UTP version) | Inquiry |
| PDRN-003 | dsRNA control (Pseudo-UTP version) | Inquiry |
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