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Protein and Proteomics

protocols
Protocol

Bacterial Total Protein and Membrane Protein Extraction Protocol

Experiment Tags: Membrane Protein, Bacterial Total Protein, Protein Extract

Experiment Summary: This experiment describes several methods for extracting total bacterial proteins and membrane proteins.

protocols
Protocol

What is the ELISpot Assay? Complete Guide to Principle, Steps, and Uses

Experiment Tags: ELISpot Assay, Cytokine Detection, Single-Cell Immunoassay

Experiment Summary: The ELISpot (Enzyme-Linked ImmunoSpot) assay is a highly sensitive immunological technique that detects secreted proteins—such as cytokines or antibodies—at the single-cell level. It quantifies the frequency of secreting cells, offering functional insights that conventional ELISA cannot provide.