SNU-5 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SNU-5 (Seoul National University-5) is a hypotetraploid human gastric cancer cell line that was originally isolated in 1987 from metastatic ascites of a 33-year-old Asian female patient with poorly differentiated gastric cancer. The patient had previously been treated with chemotherapy such as 5-fluorouracil, doxorubicin, and mitomycin-C. The cells exhibit an adherent growth pattern and show epithelial morphology. A notable feature of the SNU-5 cell line is the presence of a point mutation in the TP53 gene. TP53 is a tumor suppressor gene that plays a crucial role in regulating cell cycle progression and preventing cancer cell formation. The TP53 mutation observed in SNU-5 cells disrupts the normal function of this tumor suppressor pathway, leading to uncontrolled cell proliferation and contributing to the development and progression of gastric cancer. Understanding the effects of TP53 mutations on SNU-5 cells could provide valuable insights into the molecular mechanisms and potential therapeutic targets of gastric cancer.
Tissue	Stomach
Disease	Gastric Carcinoma
Morphology	Epithelial
Gender	Female
Age	33 years
Product Format	Frozen
Growth Mode	Suspension, multicellular aggregates
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer Research 2. Drug Testing 3. Signal Transduction Studies 4. Genomic and Proteomic Studies 5. Molecular and Cellular Biology 6. Radiation Research
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	SNU-5 cells are often used as a peritoneal dissemination model in athymic nude mice because of their high potential for peritoneal metastasis. At the same time, studies using SNU-5 cells have found that Oridonin has a strong inhibitory effect on inhibiting c-Met phosphorylation and c-Met-dependent cell proliferation, and downregulates the expression of downstream signaling molecules including phosphorylated c-Raf, phosphorylated Erk, and phosphorylated Akt. In addition, Oridonin has significant anti-tumor activity in the SNU-5 subcutaneous xenograft model through cell reduction, and its anti-tumor activity is dose-dependent and shows a strong inhibitory effect on c-Met phosphorylation.
Characteristics
Karyotype	This is a hypotetraploid human cell line with the modal chromosome number of 87 and 88 occurring in a total of 36% of cells. Cells having higher ploidies were found at 9.6%.
Antigen Expression	Blood Type O; Rh + The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Oncogene	myc+; erb-B2+
Tumorigenic	Yes
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The culture can be maintained by adding fresh medium or changing the medium. Alternatively, the culture can be established by centrifuging the suspension and then resuspending in fresh medium. Add medium as the cell density increases. These cells tend to grow in multicellular aggregates and they may lose viability when the cell clusters break up or disperse. Therefore, cell counts and viability assays should not be performed.
Medium Renewal	Every 2 to 3 days.
Culture Medium	90% DMEM medium + 10% FBS
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

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Cat.No.	Product Name	Price
CSC-RR0577	GFP Reporter Cell Line - SNU-5	Inquiry
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CSC-RR0579	GFP/Luc Reporter Cell Line - SNU-5	Inquiry

* For research use only. Not intended for any clinical use.

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