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Recombinases including Cas9 recombinase, AsCpf1 recombinase, Cre recombinase and optimized FLP recombinase (FLPo), can cause excision/insertion, inversion, translocation and cassette exchange, and are widely used in manipulating the structure of cell genomes.
CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found within the genomes. Cas9 (CRISPR-associated protein 9) is a nuclease that can recognize and cleave specific strands of DNA guided by CRISPR sequences. CRISPR-Cas9 technology plays a wide variety of applications in basic biological research, development of biotechnology products, and treatment of diseases.
AsCpf1, a member of the Cpf1 nuclease family, is an alternative nuclease to Cas9 for CRISPR mediated and has been proved to generate efficient genome editing in mammalian cells.
Cre-lox and Flp-FRT systems are popular and powerful site-specific-recombinase (SSR) tools in genome editing.
Cre recombinase, a Type I topoisomerase from P1 bacteriophage, catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. The orientation and location of introduced loxP sites allow for Cre-mediated gene translocation, inversion, or deletion. The bacteriophage P1 Cre/loxP system has proven to be a powerful tool to manipulate gene expression in vivo using transgenic mice expressing Cre recombinase under the control of specific promoters, or local viral delivery of Cre-encoding constructs.
Based on the Cre-lox recombination principles, scientists have developed constructs to activate/inactivate genes when Cre is present. By expressing Cre at specific times or locations, you can precisely control the expression of your gene of interest. Many Cre constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre- and Cre+ cells. Recombinant adeno-associated viruses (rAAVs) are often used as vehicles for in vivo gene transfer. Currently, methods that combine Cre-knockin mice and Cre-activated AAV have been developed to achieve high-level, stable, and cell-type-specific gene expression.
The FLP-FRT system is similar to the Cre-lox system and has been widely used for generating conditional knockout mice. FLP recombinase recognizes FRT sequences flanking a genomic region of interest. FLPo is a codon optimized FLP that possesses higher FRT recombination efficiency for both in vivo and in vitro in mouse.
As a leader in AAV technology, Creative Biogene has a wide variety of recombinase AAV particles. These viral particles undergo quality control, including AAV titering, confirmation of the transfer plasmid with PCR, and purity assessment. Please click on the AAV particles below to find the product of interest. If you have any special requirements, please feel free to contact us.
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