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Nucleotides Service

Gene Synthesis and Cloning Service
Gene Mutagenesis Service
Custom Vector Construction and Modification Service
Plasmid DNA Production Service
Gene Expression Service
Oligonucleotide Synthesis Service
Antisense Oligonucleotide Service
Custom NGS Oligonucleotide Service
Delivery System in Gene Therapy
mcDNA Production Service
Epigenomics Profiling Services
HighlightsServiceCase StudySupportFAQ

Molecular biology techniques are indispensable tools in biochemistry, genetics, and biophysics. These techniques involve the manipulation and analysis of DNA and RNA, enabling groundbreaking advances in understanding genetic processes. Among these methods, gene synthesis and cloning are pivotal in synthetic biology, allowing researchers to create artificial genes and study protein function. Gene synthesis, in particular, facilitates the creation of completely synthetic double-stranded DNA molecules, free from the constraints of preexisting sequences. This method has been used to generate functional bacterial or yeast chromosomes, even ones containing up to one million base pairs.

At Creative Biogene, we provide comprehensive molecular biology services, focusing on gene synthesis & cloning, gene mutation, plasmid preparation, recombinant protein expression, oligonucleotide synthesis, and DNA methylation.

Service Highlights

Technical Capabilities

1. Custom Oligonucleotide Synthesis: Supporting the synthesis of oligonucleotides ranging from ten bases to thousands of bases, also offers a variety of modification options.

2. Versatile Vector Construction: Supporting a wide range of insert sizes (100 bp to 15 kb) for gene expression and cloning applications.

3. Advanced Epigenomics Profiling: Comprehensive analysis of major epigenetic modifications, including DNA methylation and histone modifications.

4. Multiple Expression Systems: Available expression platforms including E. coli, yeast, insect, and mammalian cells, providing flexibility for different research needs.

5. Multiple Quality Grades: Offering different grades of materials (research-grade, endotoxin-free, GMP-compliant) to meet varying research and clinical needs.

6. Flexible Delivery Options: Customized delivery schedules and formats to align with project timelines and requirements.

Professional Team

1. PhD-Level Scientists: Highly qualified researchers with advanced degrees and industry experience in molecular biology and related fields.

2. Multidisciplinary Expertise: Knowledge spanning molecular biology, biochemistry, genetics, and bioinformatics to support a wide array of research projects.

3. Dedicated Project Management: Personalized project oversight to ensure timely progress and high-quality results.

4. Regular Technical Consultation: Ongoing support and technical consultation available throughout the research process to optimize outcomes.

Core Services

Gene Synthesis and Cloning Service

Our gene synthesis service allows the creation of synthetic DNA sequences, overcoming the limitations of natural gene synthesis methods. This flexibility enables gene synthesis without predefined DNA templates, suitable for genetic engineering and protein expression.

Gene Mutagenesis Service

Gene mutagenesis is an essential tool for studying gene function and protein engineering. Our service provides a full spectrum of mutagenesis techniques, from precise point mutations to large-scale insertions or deletions.

Custom Vector Construction and Modification Service

Successfully creating genetic vectors is vital for various molecular biology applications. Our custom vector construction service supports the design and modification of vectors tailored to your specific research requirements.

Plasmid DNA Production Service

Plasmid DNA is a cornerstone of molecular biology research. Our plasmid DNA production service provides reliable, scalable solutions for various applications, from cloning and genetic research to gene therapy and vaccine development.

Gene Expression Service

Creative Biogene offers comprehensive protein production services using both cell-based systems and cell-free systems. These services can generate high-quality proteins at scales ranging from micrograms to grams.

Oligonucleotide Synthesis Service

Oligonucleotides are integral to a variety of molecular biology techniques. Our oligonucleotide synthesis service offers unmodified and custom-modified oligos for diverse applications, from PCR and sequencing to gene editing.

Antisense Oligonucleotide Service

Antisense oligonucleotides (ASOs) offer a powerful approach to gene expression regulation by binding to complementary RNA targets, thereby inducing their degradation. This method holds significant potential for treating diseases such as cancer, ALS, Duchenne muscular dystrophy, and spinal muscular atrophy.

Custom NGS Oligonucleotide Service

Next-generation sequencing (NGS) relies heavily on the quality of oligonucleotides, which are critical for generating reliable sequencing data. Our custom NGS oligonucleotide service ensures the production of high-quality sequences, optimized for various sequencing applications.

Delivery System in Gene Therapy

Efficient gene delivery systems are crucial in gene therapy to ensure that genetic material reaches the target cells effectively. Our service encompasses both viral and non-viral methods, providing optimal solutions for gene therapy applications.

Epigenomics Profiling Services

Epigenomic modifications play a key role in gene regulation. Our epigenomics profiling services provide in-depth analysis of DNA methylation, histone modifications, and chromatin remodeling to advance your research into gene expression regulation.

Client Case Studies & Research Outcomes

Case Study 1

CTLA-4 insufficiency is a genetic immune disorder characterized by an impaired ability to regulate immune responses, often resulting in severe autoimmune diseases. A promising therapeutic approach is autologous T-cell gene therapy, which avoids the complications associated with allogeneic hematopoietic stem cell transplantation. Researchers in this study developed a homology-directed repair (HDR) gene-editing strategy to restore CTLA-4 function by inserting the CTLA-4 cDNA into its genomic locus in primary human T cells. This innovative approach regulated CTLA-4 expression in CD4+ T cells and restored functional transendocytosis of CD80 and CD86, key immune regulatory proteins. Experiments with patient-derived T cells demonstrated successful protein expression recovery, while in vivo studies using gene-corrected T cells in CTLA-4−/− mice showed the potential to prevent lymphoproliferation and ameliorate immune dysregulation.

The researchers utilized Creative Biogene's Oligonucleotide Synthesis Service to obtain high-quality small double-stranded oligonucleotides (dsODNs) essential for HDR editing. After synthesis, the dsODNs were integrated into RNP complexes for T-cell editing. Genomic DNA samples, post-editing, were shipped to Creative Biogene, where advanced next-generation sequencing (NGS) and data analysis were conducted.

Figure 1 illustrates the CRISPR-Cas9 approach for targeting the CTLA-4 gene locus, displaying the mutational landscape of CTLA-4 insufficiency, the design of the homology-directed repair donor, and the average gene repair efficiency across multiple healthy donors. (doi: 10.1126/scitranslmed.abn5811)Figure 1. The researchers utilized CRISPR-Cas9 to target the CTLA-4 locus and repair a point mutation using HDR. High-quality dsODNs synthesized by Creative Biogene facilitated precise gene editing, while next-generation sequencing verified HDR efficiency, achieving an average GFP expression rate of 55.83%. (Fox TA, et al., 2022)

Case Study 2

ABI1, an adaptor protein, regulates actin polymerization and cell signaling pathways. Dysregulation of ABI1 has been implicated in cancer development, influencing inflammation and cell survival processes. To explore ABI1's functions, the researchers employed proximity-dependent labeling (PDL) combined with mass spectrometry to map its interactome. Using a novel data filtering strategy, they identified 212 proximal interactors, including components of the WAVE2 complex and the TAK1-NF-κB signaling pathway. Functional assays further revealed that ABI1 modulates TAK1/RIPK1-dependent apoptosis, offering new insights into its role in cancer biology.

The researchers utilized Creative Biogene's Gene Synthesis and Cloning Service to synthesize the pcDNA3.1(+) construct containing the TurboID linker and ABI1 sequence. This construct was crucial for generating MSCV-IRES-GFP retroviruses, enabling precise expression of ABI1 in NIH/3T3 cells.

Figure 2 developed MSCV-TurboID vectors for ABI1 proximity labeling, established stable cell lines through single-cell sorting, and characterized cellular behaviors to prepare for comprehensive protein interaction mapping using PDL/MS. (doi: 10.1002/1878-0261.13374)Figure 2. The researchers developed ABI1 proximity-dependent labeling (PDL) tools using MSCV-TurboID vectors. These constructs facilitated retroviral transduction of NIH/3T3 cells, enabling the generation of GFP-positive cell lines for interactome analysis, wound-healing assays, and EdU incorporation studies. (Petersen M, et al., 2023)

Case Study 3

Verticillium wilt of olive (VWO), caused by Verticillium dahliae, severely affects olive crops by disrupting vascular function. Tolerant cultivars offer an effective management strategy, but understanding their root-level defense mechanisms remains incomplete. Current research highlights structural, genetic, biochemical, and physiological differences between tolerant and susceptible cultivars, shedding light on how tolerance is mediated at multiple mechanistic levels.

The researchers investigated six olive cultivars with varying VWO tolerance, focusing on root architecture, lignin content, and defense-related gene expression. Using primers custom-designed and synthesized by Creative Biogene, they conducted time-course qPCR analyses to evaluate the expression of genes involved in lignin biosynthesis (e.g., C4H and CO-MT), fungal cell wall degradation (β-1.3-glucanase), and innate immunity activation (BAK1 and WRKY5). Their findings revealed that tolerant cultivars exhibited higher basal lignin levels, less branched roots, and a rapid genetic response post-inoculation.

Figure 3 depicts the time-course expression patterns of β-1,3 glucanase and BAK1 genes in control and Verticillium dahliae-inoculated plants, highlighting gene expression differences between tolerant and susceptible cultivars with statistical significance markers. (doi: 10.3389/fpls.2022.863055)Figure 3. The researchers employed a time-course gene expression analysis comparing β-1,3 glucanase and BAK1 gene expressions in control and Verticillium dahliae-inoculated plants across tolerant and susceptible cultivars. (Cardoni M, et al., 2022)

Service Support

Creative Biogene is committed to providing exceptional customer support throughout every stage of your project. From initial consultation to final delivery. Whether you're conducting basic research or developing therapeutic applications, we are your trusted partner in molecular biology. Contact our support team today to discover how Creative Biogene can be a valuable resource and partner for your organization.

Quality Assurance

  • Stringent quality control processes
  • Multiple QC steps in all workflows
  • Validated antibodies and reagents
  • Comprehensive documentation and reporting

FAQ

Q: What is the turnaround time for your gene synthesis service?

A: The standard gene synthesis service typically has a turnaround time of 15-25 business days. For sequences longer than 3 kb or those with complex structures, it may take longer. We also offer expedited services for an additional fee.

Q: What scale can your plasmid DNA production service reach?

A: We offer plasmid DNA preparation services ranging from microgram to gram scales. The specific scales are:

  • Research grade (100 μg-1 g)
  • Endotoxin-free grade (100 μg-1 g)
  • Industrial scale (>100 mg)

Each level strictly follows corresponding quality control standards.

Q: Does your gene mutation service include validation experiments?

A: Yes, our gene mutation service includes complete validation experiments, such as:

  • PCR validation
  • Sequencing validation
  • Restriction enzyme digestion validation (if applicable)

Detailed validation reports are provided for all mutants.

Q: Can your gene expression service perform time-course analysis?

A: Yes, we offer flexible experimental design options, including expression profiling at multiple time points. Sampling times can be customized based on client needs, and we provide full data analysis and visualization service.

Publications
  1. Petersen M, Chorzalska A, Pardo M, et al. Proximity proteomics reveals role of Abelson interactor 1 in the regulation of TAK1/RIPK1 signaling[J]. Molecular Oncology, 2023.
  2. Chouchene L, Kessabi K, Gueguen M M, et al. Interference with zinc homeostasis and oxidative stress induction as probable mechanisms for cadmium-induced embryo-toxicity in zebrafish[J]. Environmental Science and Pollution Research, 2022, 29(26): 39578-39592.
  3. Fox T A, Houghton B C, Petersone L, et al. Therapeutic gene editing of T cells to correct CTLA-4 insufficiency[J]. Science Translational Medicine, 2022, 14(668): eabn5811.
  4. Cardoni M, Gómez-Lama Cabanás C, Valverde-Corredor A, et al. Unveiling differences in root defense mechanisms between tolerant and susceptible olive cultivars to Verticillium dahliae[J]. Frontiers in Plant Science, 2022, 13: 863055.
* For research use only. Not intended for any clinical use.
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