Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Creative Biogene specializes in providing high-quality adenovirus vector services, encompassing the construction, amplification, and purification of replication-deficient adenoviruses and helper-dependent adenoviruses (HDAd), supporting various serotypes including Ad5 and Ad5/F35. Leveraging our advanced QVirus™ platform, we have established mature and stable methodologies for vector construction, in vitro recombination, cellular packaging, recombinant adenovirus amplification, and gradient ultracentrifugation purification, complemented by systematic quality control and identification processes to ensure high purity and high-titer viral products.
Adenovirus (Ad) is a non-enveloped double-stranded DNA virus with a genome length ranging from 26 to 46 kilobase pairs (kb) and an icosahedral nucleocapsid structure. Since the 1980s, adenoviruses have become essential tools in gene therapy, vaccine development, and cancer treatment due to their efficient gene delivery capabilities, broad host range, and excellent genetic stability.
Several adenovirus vector-based vaccines and therapeutic products have received global regulatory approval, such as the COVID-19 vaccine (AstraZeneca's Vaxzevria) and Ebola virus vaccine (Janssen's Ad26.ZEBOV), validating the clinical value of adenovirus technology.

Based on the number of viral genes retained and dependency levels, adenovirus vector systems are generally classified into three categories:
Replication-competent Adenovirus


Replication-deficient Adenovirus
Helper-dependent Adenovirus

With the continuous development of gene therapy, biopharmaceuticals, and basic scientific research, adenovirus has become one of the most commonly used viral vectors due to its efficient transfection capability, non-integration into the host genome, and relatively large vector capacity. Creative Biogene, with years of viral vector research and production experience, has established an advanced adenovirus vector platform, providing customized adenovirus vector services covering basic research, animal experiments, preclinical studies, and commercial applications.
Service Offerings
Fiber-Modified Ad5-RGD Adenovirus Production

The Ad5-RGD adenovirus features RGD peptide insertions in the fiber protein to enhance infection efficiency, especially in tumor-targeting applications.
Fiber-Modified Ad5/35 Adenovirus Production

The Ad5/35 chimeric adenovirus combines an Ad5 backbone with Ad35 fiber to improve the transduction of hard-to-infect cells, such as hematopoietic cells.
Helper-Dependent Adenovirus (HDAd) Production

Helper-dependent adenoviruses (HDAd) remove all viral coding regions, offering up to 36 kb of transgene capacity with low immunogenicity for long-term expression.
Adenovirus Production & Purification

We provide a full adenovirus production and purification service, delivering high-titer and high-purity virus with excellent transfection performance.
Adenovirus Amplification & Purification

Our amplification and purification service supports scalable production of adenoviral vectors for both in vitro and in vivo studies.

Adenovirus titration is conducted using TCID50, qPCR, and immunofluorescence to quantify viral titers for diverse research needs precisely.
Creative Biogene has established a comprehensive adenovirus vector construction and production platform, with core modules including:
1. Vector Construction System
2. Packaging Cell Lines and Virus Production
3. Purification and Concentration Technologies
4. Technical Optimization and Innovation
Enhanced Safety
Production Efficiency Optimization
Cost-Benefit Analysis
Creative Biogene provides one-stop adenovirus services from vector design to purification delivery, with the standard process as follows:

Creative Biogene has established a comprehensive virus quality control process, covering the following key indicators:
| Test Item | Method | Acceptance Criteria |
| Titer Determination | qPCR/ELISA/TCID50 | Customer-specified range |
| Transgene Expression | Western blot / qPCR / IF | Clear expression |
| Purity | SDS-PAGE / HPLC | ≥95% |
| Endotoxin Level | LAL test | <5 EU/mL |
| Sterility | Culture method | Sterile |
| Mycoplasma Detection | qPCR or culture method | Negative |
| Host Residual DNA/Protein | PicoGreen / ELISA | Compliant with standards |
Highly Customizable
Supports any sequence, multi-gene construction, reporter gene co-expression
High-Titer Virus
Routinely reaches titers ranging from 10⁹ to 10¹² vp/mL
Stringent Quality Control Standards
Covers multiple indicators including transgene expression, titer, purity, endotoxin, sterility, etc.
GMP-Compatible Platform
Meets requirements for preclinical research and IND submissions
Multiple Modification Options
RGD, fiber replacement, targeting modifications supporting personalized therapeutic research
Extensive Project Experience
Serviced hundreds of virus development projects, covering cancer, cardiovascular, neurological, metabolic, and other disease areas
If you have any questions or requirements regarding our adenovirus services, please feel free to contact our technical support team. Our professional team will provide customized adenovirus solutions to meet your research and development needs.
Which cells can adenovirus infect?
Adenovirus has broad-spectrum infection capabilities, including most mammalian cell lines (such as HEK293, HeLa, A549, U87, etc.), though infection efficiency is relatively lower for stem cells or certain primary cells.
What is the relationship between adenovirus titer and infection efficiency?
Higher titers can improve infection efficiency, but appropriate MOI (Multiplicity of Infection) selection should be considered to avoid excessive cytotoxicity.
How should adenovirus be stored?
Long-term preservation at -80°C is recommended, avoiding repeated freeze-thaw cycles; short-term storage at 4°C is acceptable before use (within 1-2 weeks), but not recommended beyond this timeframe.
Can you provide GMP-grade adenovirus?
Currently, we provide research-grade adenovirus services. For GMP production (for clinical trials or IND submissions), please contact us for customized evaluation.
What are the differences between AdMax and AdEasy systems?
| Comparison Item | AdMax System | AdEasy System |
| Shuttle Plasmid | pHBAd series | pHBAd series |
| Packaging System | AdMax | AdEasy |
| Backbone Plasmid | pBHGlox(ΔE1,3)Cre | pAdEasy-1 |
| Packaging Cells | 293A cells | 293A cells |
| Packaging Strategy | Direct transfection into 293A cells after linearization | Recombination in E. coli after linearization, followed by transfection |
| Linearization Required for Packaging | No, vector is expressed directly in 293A cells | Yes, linearization after recombination, then transfection into 293A cells |
| Titer Detection | Plaque assay | Plaque assay |
| Purification Method | CsCl density gradient centrifugation | CsCl density gradient centrifugation |
| Advantages | Simple operation, high virus output rate, short cycle | High packaging success rate, low recombination false positives |
| Disadvantages | Risk of recombination false positives | Complex packaging process, longer cycle |
Reference: