LS 174T Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The LS 174T cell line was isolated from a 58-year-old Caucasian female patient with Duke's B colorectal adenocarcinoma. LS 174T cells are frequently used in biomedical research related to colon cancer and are positive for the expression of various oncogenes, including c-myc, N-myc, H-ras, N-ras, Myb, and fos. In addition, the LS 174T cell line has abundant microvilli and vacuoles and produces carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), and mucins. LS 174T cells have been an important tool in colorectal cancer research and have provided valuable insights into the molecular mechanisms of colorectal cancer development and progression, as well as potential therapeutic targets for the treatment of this disease.
Tissue	Large intestine; Colon
Disease	Adenocarcinoma; Colorectal; Dukes' type B
Morphology	Epithelial
Gender	Female
Age	58 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug development and screening 3. Gene expression studies 4. Immunotherapy research 5. Signal transduction research 6. Biomarker identification 7. In vitro models
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The LS 174T cell line was used to create the CDX (cell line derived xenograft) LS174T xenograft mouse model. The LS 174T xenograft model can be used to study monotherapies (e.g. RNAi specific siRNA/miRNA) or to determine synergistic effects due to drug resistance (i.e. bevacizumab and immunomodulatory oligonucleotide TLR9 activation).
Characteristics
Karyotype	45,X; one X chromosome missing; no other chromosomal aberrations
Protein Expression	Colon Antigen 3 +, CEA +, p53 -, GFAP -, mRNA expression +
Oncogenes	myc +, myb + , fos +, p53 +, ras +, sis -, abl -, ros -, src -
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	Volumes given are for 75 cm² flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium required proportionally. 1. Remove and discard the medium. 2. Rinse the cell layer briefly with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette out the cells. 5. Add an appropriate aliquot of the cell suspension to a new culture vessel. 6. Incubate the culture at 37°C.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:2 to 1:4 is recommended.
Culture Medium	EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01198	Cas9 Stable Cell Line - LS 174T	Inquiry
CSC-RR00689	Luciferase Reporter Cell Line - LS 174T	Inquiry
CSC-RR00792	GFP Reporter Cell Line - LS174T	Inquiry

* For research use only. Not intended for any clinical use.

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