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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The A498 cell line is a human renal cell carcinoma line derived from a patient's renal cell carcinoma in 1975. These cells are widely used in research related to kidney cancer, particularly clear cell renal cell carcinoma. The A498 cell line, characterized by its epithelioid morphology, is a valuable model for studying the molecular and cellular mechanisms of renal carcinogenesis. These cells exhibited several features typical of renal cancer, including changes in the expression of genes involved in cell cycle regulation, apoptosis, and angiogenesis. A498 cells are particularly useful for examining altered metabolic pathways in renal cancer because they exhibit unique metabolic signatures, including changes in lipid and glucose metabolism. This makes them suitable for metabolic targeting studies, exploring how altering metabolic pathways inhibits tumor growth. In addition, A498 cells are used in drug discovery and toxicology studies to test the efficacy of new chemotherapy drugs and targeted therapies. |
| Tissue | Kidney |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 52 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | The A498 cell line is a human renal cell carcinoma cell line commonly used in research related to cancer biology, drug testing, and cancer treatment development. These cells can be used to study the mechanisms of kidney cancer development, progression, and response to treatment. The A498 cell line can also be used to screen potential drugs and test the efficacy of targeted therapies for kidney cancer. Additionally, researchers can use this cell line to study the molecular pathways involved in kidney cancer and identify potential therapeutic targets. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The A498 cell line (human kidney) was used to create the CDX (cell line derived xenograft) A498 xenograft mouse model. The A498 xenograft model is currently utilized in preclinical studies involving apoptosis thru activation of JNK pathway (e.g. YC-1), inhibitors targeting hypoxia-inducible factor (HIF; e.g. ELR510444) and anti-tumor efficacy (e.g. IL-22). |
| Characteristics | |
| Isoenzymes | PGM3, 1, PGM1, 1-2, ES-D, 2, Me-2, 1, AK-1, 1, GLO-1, 2, G6PD, B |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Twice per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:8 is recommended. |
| Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0145 | GFP Stable Cell Line-A498 | Inquiry |
| CSC-RR0612 | Luciferase Reporter Cell Line - A-498 | Inquiry |
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