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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | KG-1 is a cell line established by Koeffler and Golde in 1978, derived from the bone marrow of a male patient with erythroleukemia that developed acute myeloid leukemia (AML). KG-1 cells are mostly in the mature myeloid blast or promyelocyte stage. KG-1a is a less differentiated subline of KG-1 formed after 35 passages. KG1a does not respond to colony stimulating factors in soft agar culture and does not express Ia-like antigens. KG1a is resistant to phorbol diester-induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically, and functionally less mature than the parental KG-1. In addition, KG-1a cells are resistant to chemotherapy and natural killer cell-mediated cytotoxicity. Altogether, KG-1a cells are considered as a leukemic stem cell (LSC)-enriched cell line. The study of Chen showed that KG-1a cells were resistant to both doxorubicin and arabinoside and a combination of chemotherapy. |
| Tissue | Bone; Marrow |
| Disease | Acute myelogenous leukemia |
| Morphology | Rounded |
| Gender | Male |
| Age | 59 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug screening and development 3. Stem cell research 4. Immunotherapy studies 5. Genetic studies 6. Model for myeloid disorders |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Arsenic trioxide (ATO) can inhibit the proliferation of KG-1a cells, and induce apoptosis and cell cycle arrest. Using transcriptome sequencing technology, the study found that the key genes involved in ATO-mediated KG-1a cell killing are MYC, PCNA and MCM7. |
| Characteristics | |
| Karyotype | The stemline chromosome number is 46 (pseudodiploid), with the 2S component occurring at 5.8%. |
| Antigen Expression | HLA A30, A31, B35, Cw4 |
| Isoenzymes | G6PD, B; PGM1, 1-2; PGM3, 0; ES-D, 1; Me-2, 1; AK-1, 0; GLO-1, 2 |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Transfer the cell suspension to a sterile centrifuge tube. Collect the cells by spinning at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust the optimal cell density to between 1 - 3 x 10^5 cells/mL. Split the cells when a maximum cell density of 1 - 2 x 10^6 cells/mL is reached. |
| Medium Renewal | Every 3 days |
| Subcultivation Ratio | The ratio of 1:2 is recommended. |
| Culture Medium | IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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