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AAV Biosensors

Understanding how neuronal activity patterns in the brain correlate with complex behavior is one of the primary goals of modern neuroscience. Chemical transmission is the main way of communication between neurons, however, traditional methods of detection of neurotransmitter and neuromodulator transients in the mammalian brain lack spatiotemporal precision. Modern fluorescent biosensors for neurotransmitters and neuromodulators allow monitoring chemical transmission with millisecond precision in vivo and single cell resolution. Changes in the fluorescent biosensor brightness occur upon neurotransmitter binding and can be detected using fiber photometry, stationary microscopy and miniaturized head-mounted microscopes.

Biosensors are genetically engineered fluorescent proteins (FP) attached to an additional protein sequence that makes them sensitive to small biomolecules or other physiological intracellular processes. These biosensors are introduced to cells, tissues or organisms to detect changes by spectrum changes or fluorescent microscopy. Many biosensors allow long-term imaging and can be engineered to specifically target cellular compartments or organelles. Besides, biosensors allow signaling pathway exploration or permit the measurement of a biomolecule. They do all this while preserving both spatial and temporal cellular processes.

Delivery  of biosensors to the animal brain using AAV particles.Figure 1. Delivery of biosensors to the animal brain using AAV particles.

Viral vectors allow temporal and spatial controlled expression of genes of interest (GOI) in vitro and in vivo. One such viral vector, the recombinant adeno-associated virus (rAAV) has become a staple for gene delivery in different tissues and organs, including brain. Biosensors can be expressed in the animal brain using adeno-associated viral vectors, and their cell-specific expression can be achieved with Cre-recombinase expressing animals.

As a leader in adenoviral technology, Creative Biogene is proud to offer a range of calcium or glutamate biosensor products that come ready-to-use. Our AAV biosensors are ideal for a variety of applications, such as tracking neuronal dynamics. Our biosensors come with the choice of promoter (CMV or Syn), serotype (AAV8 or AAV9), and many have an optional DIO/FLEX system for Cre-inducible expression. Please click on any of the biosensor names below for a list of our available products.

Reference

  1. Leopold A V, et al. Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience, 2019, 13.

Browse All AAV Biosensors

Cat.No. Product Name Price
AAB0006CAG-FLEX-NES-jRCaMP1a AAV (Serotype 8)Inquiry
AAB0031pRSET.GCaMP5G(7.35) AAV (Serotype 8)Inquiry
AAB0032Syn-NES-jRGECO1b AAV (Serotype 8)Inquiry
AAB0049CAG-FLEX-GCaMP6s AAV (Serotype 9)Inquiry
AAB0066Syn-GCaMP6m AAV (Serotype 9)Inquiry
AAB0017Syn-FLEX-jGCaMP7b AAV (Serotype 9)Inquiry
AAB0051Syn-NES-jRCaMP1a AAV (Serotype 9)Inquiry
AAB0056Syn-NES-jRGECO1b AAV (Serotype 9)Inquiry
AAB0011Syn-FLEX-CaMPARI AAV (Serotype 9)Inquiry
AAB0021Syn-FLEX-NES-jRGECO1b AAV (Serotype 8)Inquiry
AAB0026Syn-FLEX-GCaMP6s AAV (Serotype 8)Inquiry
AAB0028Syn-FLEX-GCaMP6f AAV (Serotype 8)Inquiry
AAB0045CAG-FLEX-NES-jRCaMP1a AAV (Serotype 9)Inquiry
AAB0046CAG-FLEX-jGCaMP7c AAV (Serotype 9)Inquiry
AAB0060Syn-FLEX-jGCaMP7s AAV (Serotype 9)Inquiry
AAB0014CMV-iGluSnFR AAV (Serotype 9)Inquiry
AAB0024Syn-FLEX-NES-jRCaMP1a AAV (Serotype 8)Inquiry
AAB0057Syn-jGCaMP7s AAV (Serotype 9)Inquiry
AAB0058Syn-FLEX-NES-jRGECO1a AAV (Serotype 9)Inquiry
AAV00140ZAAV1-CaMKII-GCaMP6mInquiry
AAV00192ZAAV5-CAG-GCaMP6mInquiry
AAV00278ZAAV9-CAG-GCaMP6mInquiry
AAB0004CAG-FLEX-NES-jRCaMP1b AAV (Serotype 8)Inquiry
AAB0007CMV-iGluSnFR AAV (Serotype 8)Inquiry
AAB0009CAG-FLEX-GCaMP6m AAV (Serotype 8)Inquiry
AAB0039CAG-FLEX-NES-jRGECO1b AAV (Serotype 9)Inquiry
AAB0044CAG-FLEX-jGCaMP7f AAV (Serotype 9)Inquiry
AAB0054Syn-NES-jRGECO1a AAV (Serotype 9)Inquiry
AAB0064Syn-GCaMP6f AAV (Serotype 9)Inquiry
AAB0036Syn-GCaMP6s AAV (Serotype 8)Inquiry
AAB0037Syn-NES-jRGECO1a AAV (Serotype 8)Inquiry
AAB0052Syn-NES-jRCaMP1b AAV (Serotype 9)Inquiry
AAV00139ZAAV1-CAG-GCaMP6fInquiry
AAV00280ZAAV9-CaMKII-GCaMP6mInquiry
AAB0003CAG-FLEX-NES-jRGECO1a AAV (Serotype 8)Inquiry
AAB0018Syn-FLEX-GCaMP6s AAV (Serotype 9)Inquiry
AAB0043CAG-FLEX-jGCaMP7s AAV (Serotype 9)Inquiry
AAB0050Syn-FLEX-GCaMP6f AAV (Serotype 9)Inquiry
AAB0015Syn-FLEX-jGCaMP7c AAV (Serotype 9)Inquiry
AAB0016Syn-FLEX-jGCaMP7f AAV (Serotype 9)Inquiry
AAB0042CAG-FLEX-NES-jRCaMP1b AAV (Serotype 9)Inquiry
AAB0012Syn-CaMPARI AAV (Serotype 9)Inquiry
AAB0022Syn-FLEX-NES-jRGECO1a AAV (Serotype 8)Inquiry
AAB0029Syn-FLEX-CaMPARI AAV (Serotype 8)Inquiry
AAB0047CAG-FLEX-jGCaMP7b AAV (Serotype 9)Inquiry
AAB0010CAG-FLEX-GCaMP6f AAV (Serotype 8)Inquiry
AAB0061Syn-FLEX-NES-jRCaMP1a AAV (Serotype 9)Inquiry
AAB0062Syn-GCaMP6s AAV (Serotype 9)Inquiry
AAV00138ZAAV1-CAG-GCaMP6mInquiry
AAB0001CAG-GCaMP3 AAV (Serotype 8)Inquiry
AAB0008CAG-FLEX-CaMPARI AAV (Serotype 8)Inquiry
AAB0025CAG-FLEX-GCaMP6f AAV (Serotype 9)Inquiry
AAB0030Syn-CaMPARI AAV (Serotype 8)Inquiry
AAB0035Syn-NES-jRCaMP1a AAV (Serotype 8)Inquiry
AAB0055Syn-FLEX-NES-jRCaMP1b AAV (Serotype 9)Inquiry
AAB0065Syn-FLEX-NES-jRGECO1b AAV (Serotype 9)Inquiry
AAV00193ZAAV5-CAG-GCaMP6fInquiry
AAV00279ZAAV9-CAG-GCaMP6fInquiry
AAB0005CAG-FLEX-GCaMP6s AAV (Serotype 8)Inquiry
AAB0040CAG-FLEX-NES-jRGECO1a AAV (Serotype 9)Inquiry
AAB0041Syn-GCaMP6f AAV (Serotype 8)Inquiry
AAB0048Syn-FLEX-GCaMP6m AAV (Serotype 9)Inquiry
AAV00191ZAAV5-CAG-GCaMP6sInquiry
AAB0034Syn-NES-jRCaMP1b AAV (Serotype 8)Inquiry
AAB0059Syn-jGCaMP7b AAV (Serotype 9)Inquiry
AAV00194ZAAV5-CaMKII-GCaMP6mInquiry
AAB0002CAG-FLEX-NES-jRGECO1b AAV (Serotype 8)Inquiry
AAB0019CAG-GCaMP3 AAV (Serotype 9)Inquiry
AAB0020Syn-FLEX-NES-jRCaMP1b AAV (Serotype 8)Inquiry
AAB0027Syn-FLEX-GCaMP6m AAV (Serotype 8)Inquiry
AAB0053Syn-jGCaMP7f AAV (Serotype 9)Inquiry
AAB0063Syn-jGCaMP7c AAV (Serotype 9)Inquiry
AAV00137ZAAV1-CAG-GCaMP6sInquiry
AAB0013pRSET.GCaMP5G(7.35) AAV (Serotype 9)Inquiry
AAB0023CAG-FLEX-GCaMP6m AAV (Serotype 9)Inquiry
AAB0033CAG-FLEX-CaMPARI AAV (Serotype 9)Inquiry
AAB0038Syn-GCaMP6m AAV (Serotype 8)Inquiry
AAV00277ZAAV9-CAG-GCaMP6sInquiry
For research use only. Not intended for any clinical use.