Our Platform

Our platform performs gene knockout/knockin programme with CRISPR/Cas9 system, from designing gRNA constructs to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines, as well as plants, mouse and other animal models.

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sgRNA Design and Expression,
Cas9 Expression

A typical sgRNA contains 98 nucleotides (including a 20-nt target sequence) and functions as a guide in the Cas9/sgRNA nuclease complex. To improve Cas9 expression in plants, most modified Cas9 genes for genome editing have also been optimized with usage bias codons.

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Delivery of the Cas9 and sgRNA
Expression Cassettes

In CRISPR/Cas9 system, there are two critical parts: sgRNA sequence and Cas9 sequence. These two sequence could be cloned into separated plasmids or combined into one plasmids.

Considering the limited utility of protoplast system, it’s also available to transfect preassembled complexes of purified Cas9 protein and synthesized sgRNA.

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Analyze editing efficiency

The genotype of the resultant mutants must be determined before carrying out further studies.

  • Using Reporter Genes to Verify Targeted Mutations
  • Using Endonucleases to Verify Targeted Mutations
  • Using PAGE to Verify Targeted Mutations
  • Using High-Resolution Melting to Verify Targeted Mutations
  • Using High-Throughput Sequencing/ Sanger Sequencing to Determine Targeted Mutations

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Why Choose Us

We Offer Best Services & Solutions

Creative Biogene provides reliable and professional gene editing services and products by CRISPR/Cas9. At Creative Biogene, we are dedicated to meet the needs of clients with high quality products and services in a reasonable price. To date, we have succeeded in lots of custom cell line generation projects and developing more than two thousand knockout cell lines.

Excellent Platform
Experienced Scientist Team
Professional Staff
Quality Guarantee