Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HCCLM3 is a highly metastatic human hepatocellular carcinoma (HCC) cell line. It was established using the MHCC97-H cell line as the parent cell line through serial lung metastasis in nude mice. HCCLM3 is characterized by its extremely high spontaneous metastasis rate, particularly to the lungs, making it an important model for studying the molecular mechanisms of cancer invasion, epithelial-mesenchymal transition (EMT), and metastasis. It is widely used to evaluate the efficacy of anti-angiogenic drugs and targeted therapies in advanced liver cancer. |
| Tissue | Liver |
| Disease | Hepatocellular Carcinoma |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Hepatocellular carcinoma invasion and metastasis research 2. Study of Epithelial-Mesenchymal Transition (EMT) pathways 3. Anti-tumor drug screening and efficacy testing 4. Investigation of tumor angiogenesis and vascular mimicry 5. Development of orthotopic liver cancer animal models |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic and metastatic in nude mice |
| Metastatic Potential | High pulmonary metastasis rate (up to 100% in orthotopic models) |
| Expression Markers | Positive for Alpha-Fetoprotein (AFP); High expression of VEGF and TGF-beta |
| Genetic Profile | TP53 mutation; Hepatitis B virus (HBV) DNA integration |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-EDTA solution and incubate at 37°C until cells detach (usually 3 to 5 minutes). 4. Neutralize with complete culture medium and gently pipette to obtain a single-cell suspension. 5. Aliquot the cells into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | DMEM (High Glucose) supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00769 | GFP Reporter Cell Line - HCCLM3 | Inquiry |
| CSC-RR00770 | Luciferase Reporter Cell Line - HCCLM3 | Inquiry |
| CSC-RR01013 | GFP/Luc Reporter Cell Line - HCCLM3 | Inquiry |
Copyright © Creative Biogene. All rights reserved.