SNU-387 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SNU-387 was isolated in 1990 by J.-G. Park and colleagues from a Korean patient with primary hepatocellular carcinoma who had been treated with transcatheter arterial embolization using lipopolysaccharide supplemented with doxorubicin and mitomycin C. Southern blot can detect hepatitis B virus (HBV) DNA, but does not express HBV genomic RNA, and must be operated under biosafety level 2. SNU-387 cells form tumors in nude mice. The SNU-387 cell line has been widely used in scientific research, especially in oncology and hepatology.
Tissue	Liver
Disease	Pleomorphic Hepatocellular Carcinoma
Morphology	Epithelial
Gender	Female
Age	41 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer Research 2. Drug Testing and Development 3. Mechanistic Studies 4. Genetic Studies 5. Biomarker Identification 6. In Vitro Modeling 7. Signaling Pathway Analysis
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Grossly, the original tumor is a single nodule. Histologically, it is predominantly compact and trabecular. Cultured cells contain a single nucleus.
Characteristics
Karyotype	Aneuploid; modal number = 67
Antigen Expression	Blood Type O; Rh +
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes used in this protocol are for 75 cm2 flasks; for culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently remove the cells by pipetting. 5. Add an appropriate amount of the cell suspension to a new culture vessel. 6. Incubate at 37℃.
Medium Renewal	Every 2 to 3 days.
Subcultivation Ratio	The ratio of 1:3 to 1:6 is recommended.
Culture Medium	RPMI-1640 + 10% FBS + 1% P/S
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

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Cat.No.	Product Name	Price
CSC-RO0164	Cas9 Stable Cell Line - SNU-387	Inquiry
CSC-RR00715	Luciferase Reporter Cell Line - SNU-387	Inquiry

* For research use only. Not intended for any clinical use.

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