KYSE30 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	KYSE30 was established from an untreated esophageal cancer in a 64-year-old man. Tumor samples were obtained from the mucosal surface of well-differentiated squamous cell carcinoma. The cell line KYSE30 was established using tumors originally transplanted into athymic mice. The doubling time of cells in the exponential growth phase was reported to be 20.8 hours.
Tissue	Esophagus
Disease	Squamous Carcinoma
Morphology	Epithelial-like
Gender	Male
Age	64 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer biology research 2. Drug discovery and development 3. Biomarker identification 4. Personalized medicine
Shipped in	dry ice
Storage Temperature	−196°C
Additional Info	A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.
Characteristics
Virus Tested	CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested]
Mycoplasma Test	Negative
Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST and MD.
Culture Conditions and Handling
Thawing Frozen Cells	1. Thaw vials in a 37°C water bath with gentle stirring. To reduce the possibility of contamination, keep the Oring and cap out of the water. Thawing should be quick (about 2 minutes). 2. Immediately after the contents have thawed, remove the vial from the water bath and decontaminate it by immersing or spraying it in 70% ethanol. All operations thereafter should be performed under strict sterile conditions. 3. For cells that are sensitive to DMSO, it is recommended to remove the cryoprotectant immediately. Transfer the contents of the vial to a centrifuge tube containing 9.0 mL of complete medium and spin at approximately 125 x g for 5 to 7 minutes. 4. Discard the supernatant and resuspend the cell pellet in the recommended complete medium. 5. Incubate the culture in a appropriate atmosphere and temperature. NOTE: It is important to avoid over-alkalinity of the medium during cell recovery. It is recommended that the culture vessel containing the growth medium be placed in the incubator for at least 15 minutes before adding the contents of the vial to allow the medium to reach normal pH (7.0 to 7.6).
Medium Renewal	Every 2-6 days
Subcultivation Ratio	The ratio of 1:10 is recommended.
Culture Medium	RPMI 1640 + Ham's F12 (1:1) + 2mM Glutamine + 2% Fetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Cryopreservation	95% FBS + 5% DMSO (Dimethyl sulfoxide)

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Cat.No.	Product Name	Price
CSC-RO01195	Cas9 Stable Cell Line - KYSE-30	Inquiry
CSC-RR00687	Luciferase Reporter Cell Line - KYSE-30	Inquiry
CSC-RR00788	GFP Reporter Cell Line - KYSE-30	Inquiry
CSC-RT0088	DDAH2 Knockout Cell Line-KYSE30	Inquiry
CSC-RT0093	TNXB Knockout Cell Line-KYSE30	Inquiry
CSC-RT0094	CCHCR1 Knockout Cell Line-KYSE30	Inquiry
CSC-RT2585	Human CYP26B1 Knockout Cell Line-KYSE30	Inquiry
CSC-RT2589	Human FASN Knockout Cell Line-KYSE30	Inquiry
CSC-RT2648	Human MCM10 Knockout Cell Line-KYSE30	Inquiry

* For research use only. Not intended for any clinical use.

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