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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HEC-1-A is a human endometrial adenocarcinoma cell line established in 1968 from a 71-year-old patient. It is one of the earliest identified endometrial cancer cell lines and is widely used as a model for type I endometrial cancer. HEC-1-A cells exhibit epithelial cell morphology and adherent growth patterns. Because they express multiple receptors and respond to estrogen and progesterone, they are of significant value in studying the hormonal regulation of endometrial tissue. This cell line is widely used in research on cell-cell interactions, drug sensitivity, and the molecular mechanisms of uterine malignancies. |
| Tissue | Endometrium; Uterus |
| Disease | Adenocarcinoma; Endometrial Cancer |
| Morphology | Epithelial |
| Gender | Female |
| Age | 71 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Endometrial cancer biology and hormonal regulation research 2. Study of uterine epithelial cell polarity and barrier function 3. Drug screening for gynecological malignancies 4. Investigation of estrogen and progesterone receptor signaling 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Pseudodiploid; Modal number = 46 |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | KRAS mutation; PTEN mutation; MSI (Microsatellite Instability) positive |
| Receptors Expressed | Estrogen Receptor (ER); Progesterone Receptor (PR) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently pipette until the cell suspension is a single-cell suspension. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:10 is recommended |
| Culture Medium | McCoy's 5A Medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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