U14 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	U14 is a mouse cervical cancer cell line originally derived from spontaneous cervical cancer in female mice. It is one of the most widely used homology models for studying cervical cancer in immunocompetent mice. The U14 cell line is characterized by high tumorigenicity, capable of forming solid tumors with a pathological progression highly similar to human cervical squamous cell carcinoma. Because it can be studied in a homologous host, it is an important tool for researching tumor immunology, evaluating the efficacy of anti-tumor vaccines, and testing various immunotherapeutic interventions.
Tissue	Uterine Cervix
Disease	Cervical Squamous Cell Carcinoma
Morphology	Epithelial-like; Polygonal cells
Gender	Female
Age	Adult
Product Format	Frozen
Growth Mode	Adherent (also adaptable to ascites form in vivo)
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Homologous mouse models for cervical cancer research 2. Development and testing of anti-tumor vaccines 3. Research on tumor-related immunosuppression 4. Evaluation of the efficacy of traditional drugs and novel chemotherapy 5. Research on tumor angiogenesis and angiogenesis-targeted therapy
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic in syngeneic mouse strains (e.g., C57BL/6 depending on subline)
Expression Markers	Positive for epithelial markers; tumor-associated antigens
In Vivo Form	Capable of growing as solid tumors or as an ascites model
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.53 mM EDTA solution and incubate at 37°C for 2 to 5 minutes until cells detach. 4. Neutralize with complete culture medium and gently pipette to disperse cell clumps. 5. Aliquot the cell suspension into new culture containers.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended
Culture Medium	RPMI 1640 or DMEM supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 10% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RR00851	GFP Reporter Cell Line - U14	Inquiry
CSC-RR00852	Luciferase Reporter Cell Line - U14	Inquiry
CSC-RR00989	GFP/Luc Reporter Cell Line - U14	Inquiry

* For research use only. Not intended for any clinical use.

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