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|Organism||Homo sapiens, human|
|Cell Line Description||MCF-7 is a human breast cancer cell line which was first isolated in 1970 from the malignant adenocarcinoma breast tissue of a 69-year old woman. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells can carry B or C type retrovirus and are thought to represent a category 2 pathogen (P2 containment). Cells express the wild-type and variant estrogen receptors as well as progesterone receptor.|
|Tissue of Origin||Breast|
|Age||69 years adult|
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
|Applications||Cell culture/growth conditions, stable cell transfection, nucleic acid purification, protein expression, gene expression, transient transfection and RNAi|
|Shipped in||Dry ice|
|References||J Nat Cancer Inst 1973;51:1409; Cancer Res 1993;53:5882|
|Karyotype||Modal number = 82; range = 66 to 87; |
The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29-34 marker chromosomes per S metaphase; 24-28 markers occurred in at least 30% of cells, and usually one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic.
|Genes Expressed||Insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5|
|Antigen Expression||Blood Type O; Rh+|
|Receptors Expression||Estrogen receptor, expressed|
|Oncogene||The cells express the WNT7B oncogene.|
|Comments||MCF-7 is a widely studied epithelial cancer cell line derived from breast adenocarcinoma. The cell has characteristics of differentiated mammary epithelium. MCF7 cells can be used for detecting MAPK and PI3K involvement, along with easy detection of ERK and Akt phosphorylation. The growth of MCF7 cells is inhibited by tumor necrosis factor α (TNF-α). Secretion of IGFBP's can be modulated by treatment with anti-estrogens.|
|Culture Conditions and Handling|
|Culture Medium||In order to make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human recombinant insulin; fetal bovine serum to a final concentration of 10%.|
Volumes are given for a 75 cm2 flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes.
|Subcultivation Ratio||The ratio of 1:3 to 1:6 is recommended.|
|Fluid Renewal||2 to 3 times per week|
|Freeze Medium||Complete growth medium 95%; DMSO, 5%|
|Atmosphere||Air, 95%; carbon dioxide (CO2), 5%|
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|CSC-RT2086||Human LOXL2 Knockout Cell Line-MCF7||Inquiry|
|CSC-RT2087||Human ARF1 Knockout Cell Line-MCF7||Inquiry|
|CSC-RT2088||Human PDE3B Knockout Cell Line-MCF7||Inquiry|
|CSC-RT2655||Human OVOL2 Knockout Cell Line-MCF7||Inquiry|
|CSC-RO0595||Human CYP19A1 Stable Cell Line - MCF7||Inquiry|
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