MCF7 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	MCF-7 is a human breast cancer cell line which was first isolated in 1970 from the malignant adenocarcinoma breast tissue of a 69-year old woman. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells can carry B or C type retrovirus and are thought to represent a category 2 pathogen (P2 containment). Cells express the wild-type and variant estrogen receptors as well as progesterone receptor.
Tissue of Origin	Breast
Age	69 years adult
Gender	Female
Ethnicity	Caucasian
Cell Type	Epithelial
Product Format	Frozen
Growth Mode	Adherent
Applications	Cell culture/growth conditions, stable cell transfection, nucleic acid purification, protein expression, gene expression, transient transfection and RNAi
Primary	No
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Subculturing	Volumes are given for a 75 cm² flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes. Remove culture medium to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5-15 minutes). Cells that are difficult to detach can be placed at 37°C to facilitate dispersal. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at 125 x g for 5-10 minutes. Discard the supernatant. Resuspend the cell pellet in the fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	The ratio of 1:3 to 1:6 is recommended.
Fluid Renewal	2 to 3 times per week
Freeze Medium	Complete growth medium 95%; DMSO, 5%
Culture Temperature	37°C
Atmosphere	Air, 95%; carbon dioxide (CO₂), 5%

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Cat.No.	Product Name	Price
CSC-DC013461-1	Human ROCK1 Knockdown Cell Line - MCF7	Inquiry
CSC-DC013462-1	Human ROCK2 Knockdown Cell Line - MCF7	Inquiry
CSC-RO0028	Cas9 Stable Cell Line - MCF-7	Inquiry
CSC-RO0595	Human CYP19A1 Stable Cell Line - MCF7	Inquiry
CSC-RR0049	Luciferase Reporter Stable Cell Line-MCF-7	Inquiry
CSC-RR0107	GFP Stable Cell Line-MCF-7	Inquiry
CSC-RR0214	Luc/GFP Reporter Cell Line-MCF7	Inquiry
CSC-RR0301	Nrf2-Luc Reporter Cell Line-MCF7	Inquiry
CSC-RR0383	NFkB Luciferase Reporter Cell Line - MCF7	Inquiry
CSC-RT2086	Human LOXL2 Knockout Cell Line-MCF7	Inquiry
CSC-RT2087	Human ARF1 Knockout Cell Line-MCF7	Inquiry
CSC-RT2088	Human PDE3B Knockout Cell Line-MCF7	Inquiry
CSC-RT2655	Human OVOL2 Knockout Cell Line-MCF7	Inquiry

* For research use only. Not intended for any clinical use.

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