MIA PaCa-2 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	MIA PaCa-2 is a human pancreatic cancer cell line widely used in pancreatic cancer research and therapeutic development. In 1977, MIA PaCa-2 cells were derived from cancer in a 65-year-old man. These cells display CK5.6, AE1/AE3, E-cadherin, vimentin, chromogranin A, synaptophysin, SSTR2, and NTR1, but not CD56. This cell line is commonly used as a model system in cancer research to study the properties and behavior of pancreatic cancer cells and for drug screening and development.
Tissue	Pancreas
Disease	Carcinoma
Morphology	Epithelial
Gender	Male
Age	65 years
Product Format	Frozen
Growth Mode	Adherent, single cells and loosely attached clusters
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	3D cell culture Studying cancer biology Drug screening Cell signaling studies Gene expression analysis
Shipped in	Dry ice
Storage Temperature	−196°C
Additional Info	Studies of MIA PaCa-2 physiology could help elucidate the oncogenic mechanisms of pancreatic cancer, aid in the development of cancer cell lysates targeting IgG production, and enhance quantum dot-dependent drug delivery methods.
Characteristics
Karyotype	This is a subtriploid human cell line. The most common number of chromosomes is 61. There are typically 16 to 20 marker chromosomes in a cell. Some normal chromosomes are missing.
Genes Expressed	Human colony-stimulating factor, subclass I (CSF-I); plasminogen activator
Mycoplasma Test	Negative
Bacteria Test	Negative
Yeast Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add fresh culture medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:3 to 1:8 is recommended.
Culture Medium	DMEM + 10% h.i. FBS + 2.5% horse serum
Culture Conditions	Atmosphere: air, 90%; carbon dioxide (CO₂), 10%; Temperature: 37°C
Cryopreservation	Frozen with 70% medium, 20% FBS, 10% DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0909	Human CD274 Stable Cell Line - MIA PaCa-2	Inquiry
CSC-RO1003	Human CLDN18-A2 Stable Cell Line - MIA PaCa-2	Inquiry
CSC-RR0322	Luciferase Reporter Cell Line - MIA PaCa-2	Inquiry
CSC-RR0434	GFP Stable Cell Line - MIA PaCa-2	Inquiry
CSC-RR0650M	RFP Reporter Cell Line - MIA PaCa-2	Inquiry
CSC-RR0651M	RFP/Luc Reporter Cell Line - MIA PaCa-2	Inquiry
CSC-RT2640	Human IL-17RA Knockout Cell Line - MIA PaCa-2	Inquiry
CSC-RT2716	Human VHL Knockout Cell Line-MIA PaCa-2	Inquiry

* For research use only. Not intended for any clinical use.

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