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Induced pluripotent stem cells (iPSCs) are pluripotent stem cells derived from somatic cells through the introduction of pluripotency-associated genes or reprogramming factors. iPSC technology allows the development of diverse human cell types, with resulting tissues closely resembling the donor's cells, minimizing immune rejection. This makes iPSCs valuable in drug development and transplantation medicine. Reprogramming factors, including OCT3/4, SOX2, NANOG, LIN28, c-Myc, and KLF4, are introduced into somatic cells using lentiviruses. Creative Biogene offers high-titer, prepackaged lentiviral particles to expedite iPSC generation. Explore our product list for the right reporter lentivirus for your needs. If you have any special requirements, please feel free to contact us!
Key Features of Our iPS Lentiviral Particles
iPS Lentiviral Particles Product List
The lentiviral iPSC vectors efficiently introduce reprogramming factors into somatic cells to induce pluripotency with genomic integration for stable expression. The lentiviral integration also allows efficient reprogramming and selection of iPSCs. The principle involves the transduction of somatic cells with cocktails of the transcription factor-encoding lentiviruses to initiate reprogramming cascades. Creative Biogene's products can promptly support your research in the following areas:
Case Study 1
The researchers devised an innovative strategy to streamline the reprogramming of somatic cells by utilizing a single virus containing reprogramming factors delivered through 2A "self-cleaving" peptides. This approach reduces the risk of insertional mutagenesis associated with the viral-based delivery of reprogramming factors, addressing a major concern in induced pluripotent stem (iPS) cell technology. Up to four reprogramming factors can be efficiently expressed from a single virus, enabling the generation of iPS cells in both embryonic and adult somatic mouse cells. Notably, a single proviral copy is demonstrated to be sufficient for generating iPS cells from mouse embryonic fibroblasts. Furthermore, the researchers successfully applied this approach to generate human induced pluripotent stem (hiPS) cell lines from human keratinocytes, showcasing the versatility and efficiency of this single-virus polycistronic method in reprogramming human somatic cells.
Figure 1. The researchers employed lentiviral vectors with 2A peptides for efficient polycistronic expression of Oct4, Sox2, c-Myc, and Klf4 in the generation of mouse iPS cells. (Carey BW, et al., 2009)
Case Study 2
The circadian clock orchestrates tissue-specific clock-controlled genes, governing tissue homeostasis. In articular cartilage, disturbances in the circadian rhythm correlate with a pro-catabolic condition. The researcher employed lentiviral vectors to engineer murine-induced pluripotent stem cells (miPSCs) for the development of genetically modified cartilage. This innovative approach aimed to protect the circadian clock in the presence of inflammation, preserving tissue homeostasis and offering potential therapeutic strategies for diseases like osteoarthritis. Results demonstrated that engineered cartilage, resistant to inflammation-induced degradation, maintained circadian rhythms, showcasing a synthetic gene circuit's ability to support daily rhythms in cartilage. This work holds promise for future cell-based therapies to safeguard the circadian clock in tissue engineering applications.
Figure 2. The researcher utilized lentivirus with Per2-Luc or Bmal1-Luc for monitoring circadian rhythms during chondrogenesis in various cell stages, revealing circadian clock development in tissue-engineered cartilage. (Pferdehirt L, et al., 2022).
Q: What is the primary purpose of iPS Lentiviral Particles in research involving induced pluripotent stem cells (iPSCs)?
A: iPS Lentiviral Particles are designed for the delivery and expression of genes associated with iPSCs, facilitating the reprogramming of differentiated cells into iPSCs. Lentiviral delivery offers stable and prolonged expression of reprogramming factors, increasing the efficiency of iPSC generation compared to transient methods.
Q: How do iPS Lentiviral Particles contribute to the process of cellular reprogramming into induced pluripotent stem cells?
A: These particles carry genes essential for inducing pluripotency, enabling the reprogramming of differentiated cells into iPSCs, a process crucial for regenerative medicine and cellular differentiation studies.
Q: Which specific genes are commonly delivered by iPS Lentiviral Particles for cellular reprogramming?
A: iPS Lentiviral Particles typically deliver genes such as Oct4, Sox2, Klf4, and c-Myc, known as the Yamanaka factors, which are key players in inducing pluripotency.
Q: In what experimental contexts are iPS Lentiviral Particles particularly valuable?
A: These particles are valuable in experiments focused on cellular reprogramming, studying pluripotency, and generating iPSCs for regenerative medicine applications.
| Cat.No. | Product Name | Price |
|---|---|---|
| LV00939Z | Human EF1A-KLF4 lentiviral particles | Inquiry |
| LV00940Z | LIN28 lentiviral particles | Inquiry |
| LV00942Z | Human NANOG lentiviral particles | Inquiry |
| LV00944Z | Human EF1A-SOX2 lentiviral particles | Inquiry |
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