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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The HuH-7 cell line was established in 1982 from a well-differentiated hepatocyte-derived carcinoma cell line originally derived from a liver tumor of a 57-year-old Japanese man. This cell line was established by Nakabayshi, H. and Sato, J. Most Huh-7 cells have chromosome numbers between 55 and 63 (pattern 60) and are highly heterogeneous. These cells adhere to the surface of a flask or plate and typically grow as a 2D monolayer. Huh-7 is highly sensitive to hepatitis C virus (HCV) and is often used as a model to study liver cancer and HCV. This cell line can be used in HCV replicon systems, allowing the production of infectious HCV particles in vitro and the development of drugs against HCV. |
| Tissue | Liver |
| Disease | Hepatocellular carcinoma |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | 57 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Viral hepatitis research 2. Drug metabolism/pharmacology studies 3. Cancer research 4. Gene expression studies 5. 3D cell culture |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Huh7 cells have played an important role in hepatitis C research. Until 2005, it was impossible to culture hepatitis C in the laboratory. The introduction of the Huh7 cell line allows screening of drug candidates against laboratory-grown hepatitis C virus and allows the development of new drugs against hepatitis C. |
| Characteristics | |
| Cytogenetic Information | The HuH7 cell line is characterized by a heterogeneous chromosome number, typically ranging from 55 to 63, reflecting genetic variability within the cell population. |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, cells were gently mixed with 10 ml of medium to resuspend and centrifuged at 300xg for 3 min. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | Every 3 days |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Cells should be suspended in specially designed freezing medium, such as CM-1. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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