Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | bEnd.3 is a mouse brain capillary endothelial cell line derived from T antigen-converted polyomaviruses in BALB/c mice. Because it maintains many morphological and functional characteristics of primary brain endothelial cells, it is currently the most widely used in vitro model of the blood-brain barrier (BBB). bEnd.3 cells can form a tightly connected fused monolayer and express BBB-specific transport proteins and receptors. This cell line is an important tool for studying cerebral vascular permeability, leukocyte adhesion, and the transport of drugs and nutrients across the BBB. |
| Tissue | Brain; Cerebral Cortex |
| Cell Type | Endothelial |
| Morphology | Polygonal; Epithelial-like |
| Gender | Not specified |
| Age | 6 weeks |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. In vitro blood-brain barrier (BBB) permeability and transport studies 2. Neuroinflammation and leukocyte-endothelial cell interactions studies 3. Cerebral angiogenesis and signal transduction studies 4. Screening for targeted drug delivery to the central nervous system (CNS) 5. Studies on the interaction between viruses or bacteria and brain microvascular endothelial cells |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, forms hemangiomas in syngeneic mice |
| Specific Function | Maintains BBB characteristics (expression of ZO-1, Occludin, and Claudin-5) |
| Transporters | Expresses GLUT1 (glucose transporter) and P-glycoprotein (P-gp) |
| Expression Markers | Positive for von Willebrand factor (vWF) and uptake of acetylated LDL |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 3 to 8 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:10 is recommended |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with high glucose (4.5 g/L), supplemented with 10% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO01437 | OVAL Stable Cell Line - bEnd.3 | Inquiry |
| CSC-RO01690 | GFP/OVAL Stable Cell Line - bEnd.3 | Inquiry |
| CSC-RO01943 | Luc/OVAL Stable Cell Line - bEnd.3 | Inquiry |
| CSC-RO02196 | GFP/Luc/OVAL Stable Cell Line - bEnd.3 | Inquiry |
| CSC-RR00738 | Luciferase Reporter Cell Line - bEnd.3 | Inquiry |
| CSC-RR00739 | GFP Reporter Cell Line - bEnd.3 | Inquiry |
Copyright © Creative Biogene. All rights reserved.