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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The NALM6 cell line was derived from cultures of the blood of a 19-year-old male patient diagnosed with non-T, non-B acute lymphoblastic leukemia (ALL) during relapse. These cultures generated eight "leukemic" cell lines and two "normal B" cell lines. Interestingly, when cultured, the leukemic blasts displayed a unique membrane phenotype characterized by the presence of SmIg (lambda and mu chain immunoglobulins), Ia-like B-cell-associated antigen (Ia), and common forms of ALL-specific antigens (cALL). The Nalm6 cell line is a reliable model to study the complex nature of acute leukemia. It has facilitated the study of gene expression profiles involved in glycolysis, lipid and carbohydrate metabolism, and the mTORC1 pathway, highlighting metabolic reprogramming in leukemic cells. Furthermore, the use of this cell line in reverse genetics and whole transcriptome analyses has helped to dissect the complex molecular networks driving leukemia progression and drug resistance. |
| Tissue | Peripheral blood |
| Disease | Acute lymphoblastic leukemia ALL |
| Morphology | Lymphocyte-like |
| Gender | Male |
| Age | 19 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug testing and development 3. Immunotherapy studies 4. Genetic studies 5. Signal transduction research 6. Cell-based assays 7. High-throughput screening |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The sensitivity of the NALM6 cell line to cytotoxic agents and its role in elucidating the function of homologous recombination (HDR) repair is of particular interest, especially with regard to the ability of HDR cells to correct DNA damage. Studies utilizing the NALM6 cell line, including studies of clonal variants such as clone G5 and drug-resistant cell lines such as C9 with a high frequency of HPRT mutations or with a resistance index, have provided insights into leukemia heterogeneity. |
| Characteristics | |
| Karyotype | Human near diploid karyotype |
| Immunology | CD3 -, CD10 +, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, cyIgM +, smIgM -, sm/cykappa -, sm/cylambda -, sm/cyIgG - |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Maintain the culture by regularly adding or replacing the medium. Start the culture at 2 x 10^5 cells/mL and maintain the cell concentration in the range of 1 x 10^5 to 1 x 10^6 cells/mL for optimal growth. |
| Medium Renewal | Add fresh medium every 2 to 3 days (depending on cell density). |
| Doubling Time | 35 to 40 hours |
| Culture Medium | 90% RPMI 1640 + 10% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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