Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The SH-SY5Y cell line is a human neuroblastoma cell line commonly used in neuroscience research to study neurodevelopment, neurodegenerative diseases, and neurotoxicity. The SH-SY5Y cell line was derived from a bone marrow biopsy of a 4-year-old female with neuroblastoma. SH-SY5Y was cloned from the bone marrow biopsy-derived line SK-N-SH by the laboratory of June Biedler and first reported in 1973. A neuroblast-like subclone of SK-N-SH, termed SH-SY, was subcloned as SH-SY5, and a third subcloning resulted in the SH-SY5Y line, first described in 1978. |
| Tissue | Bone |
| Disease | Neuroblastoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 4 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | High-throughput screening Immunology Neuroscience 3D cell culture |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | SH-SY5Y cells have an adrenergic phenotype but also express dopaminergic markers and therefore have been used to study Parkinson's disease, neurogenesis, and other features of brain cells. |
| Characteristics | |
| Antigen Expression | Blood Type A; Rh+ |
| Tumorigenic | Yes, tumors form in nude mice. |
| Mycoplasma Test | Negative |
| Cytogenetic Information | The SH-SY5Y line is genetically female, with two X chromosomes and no Y chromosome. SH-SY5Y cells have an abnormal chromosome 1 with an extra copy of the 1q segment, known as trisomy 1q. |
| Culture Conditions and Handling | |
| Subculturing | Cells typically grow in tissue culture in two different ways. Some grow into clumps of cells that float in the culture medium, while others form clumps that stick to the dish. SH-SY5Y cells can spontaneously switch between two phenotypes (neuroblast-like cells and epithelial-like cells) in vitro. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add fresh complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Every 4 to 7 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:5 is recommended. |
| Culture Medium | The most commonly used growth mixture is a 1:1 mixture of DMEM and Ham's F12 medium with 10% supplemented fetal bovine serum. DMEM typically contains 3.7 g/L sodium bicarbonate, 2 mM L-glutamine, 1 mM sodium pyruvate, and 0.1 mM non-essential amino acids. |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RG0030 | Human TACR3-tGFP Stable Cell Line-SHSY5Y | Inquiry |
| CSC-RG0035 | Human TACR1-tGFP Stable Cell Line-SHSY5Y | Inquiry |
| CSC-RG0036 | HumanDRD1-tGFP Stable Cell Line-SHSY5Y | Inquiry |
| CSC-RG0037 | HumanDRD3-tGFP Stable Cell Line-SHSY5Y | Inquiry |
| CSC-RG0038 | Human DRD5-tGFP Stable Cell Line-SHSY5Y | Inquiry |
| CSC-RO0181 | Cas9 Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO02534 | Human SNCA Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO02535 | Human SNCA-A53T Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO02551 | Human APP Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO1154M | Human MAPT/G272V-GFP Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO1155M | Human MAPT/K280del-GFP Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO1156M | Human MAPT/P301L-GFP Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO1157M | Human MAPT/G272V/K280del-GFP Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO1158M | Human MAPT/G272V/P301L-GFP Stable Cell Line - SH-SY5Y | Inquiry |
| CSC-RO1159M | Human MAPT/K280del/P301L-GFP Stable Cell Line - SH-SY5Y | Inquiry |
Copyright © Creative Biogene. All rights reserved.