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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HGC-27 cells are derived from human gastric adenocarcinoma, derived from metastatic lymph nodes of patients diagnosed with undifferentiated carcinoma. These cells exhibit polygonal or short spindle morphology and form a monolayer when adhered to glass surfaces, retaining the undifferentiated features of the original tumor. Enzyme activity in HGC-27 cells is generally low or negative, with the exception of ATPase, lactate dehydrogenase, and leucine aminopeptidase. Studies using HGC-27 have provided insights into cellular processes such as epithelial-mesenchymal transition (EMT), a key event in cancer metastasis. In addition, this cell line has been used to explore receptor signaling pathways and their effects on cancer cell behavior, providing key data for the development of targeted therapies. |
| Tissue | Gastric |
| Disease | Gastric adenocarcinoma |
| Morphology | Epithelial-like, polygonal or short spindle-shaped |
| Gender | Unspecified |
| Age | Unspecified |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug development and screening 3. Biological pathway analysis 4. Biomarker discovery |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The metabolism of the HGC-27 cancer cell line was significantly altered compared to normal gastric cells. This was manifested by an increased production of methyl ketones containing an odd number of carbons. Within this group of species, three were produced exclusively by this cell line, namely 2-undecanone, 2-tridecanone, and 2-heptadecanone. Another interesting feature of the HGC-27 volatile footprint was the reduced content of alcohols and esters. |
| Characteristics | |
| Tumorigenic | Yes |
| Protein Expression | p53 negative |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask already containing fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Culture Medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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