Plant-based Protein Production Service

Creative Biogene has established various plant-based protein expression systems to provide recombinant protein production service for global clients. Using our state-of-art platform, we offer a complete service that begins from a specific gene to the purified protein, as well as different options for protein expression systems. We devote to accelerate clients' research in the development and manufacture of therapeutic drugs, vaccines, and diagnostics.

Plants provide an inexpensive and simple system for the production of valuable recombinant proteins on large scale. Comparing with the conventional microbial and animal cell culture systems, plant-based system has numerous of clear advantages such as flexibility to rapidly scale up, reduced input costs, appropriate post-translational modifications, and absence of potential risks of contamination with human pathogens. In addition, Plant production systems include microalgae, cells, hairy roots, moss, and whole plants with both stable and transient expression. Platform diversity provides flexibility in expressing novel recombinant proteins, enables customizing and meeting scale-up needs, and provides opportunity for oral-based delivery of proteins.

Figure 1. Various plant-based expression platforms for the production of recombinant proteins.

Workflow

Gene synthesis, codon optimization, and cloning into suitable vectors.
Bioreactor growth
Gene transfer
Productivity and stability analysis of selected transformants.
Bioreactor harvest and extraction
Protein purification and characterization.
Protein stability formulations.

Plant-based protein expression services offered by Creative Biogene

Tobacco transient and stable expression system
Lettuce transient and stable expression system
Rice endosperm-specific expression system
Plant cell suspension-based expression system
Plant hairy roots-based expression system
Microalgae expression system
Wheat germ cell-free expression system

Benefits of plant-based expression system

Rapid throughput: we can move a program from Agrobacterium infiltration to protein production in as little as two weeks.
Low cost: no expensive, labor-intensive, and costly cell line development
Scalable: no time-consuming scale-up challenges
Safe: mammalian viruses can't grow in plants, so Creative Biogene's plant-based production platform minimizes contamination risks.

Creative Biogene utilizes our plant-based expression systems to offer services in custom design, expression and purification of client proteins for a range of applications, including therapeutic drugs, vaccine development, diagnostics, biophysical analysis and structural biology. Our plant-based protein expression system promotes extremely high-level and rapid production of proteins without viral replication and is efficient, safe and simple to use. For more information, please feel free to contact us.

References:

Burnett, M. (2020). Therapeutic recombinant protein production in plants: challenges and opportunities. Plants, People, Planet, 2(2).
Xu, JF. (2012). Green factory: plants as bioproduction platforms for recombinant proteins. Biotechnology Advances, 30(5), 1171-1184.

* For research use only. Not intended for any clinical use.

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