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microRNAs (miRNAs) are endogenous small regulatory RNAs that play critical roles in gene expression, cell fate determination, metabolism, and disease progression. To investigate miRNA function through gain-of-function (overexpression) or loss-of-function (inhibition) studies, chemically modified miRNA mimics (agomirs) and antisense inhibitors (antagomirs) have become widely used and highly efficient tools. Creative Biogene provides one-stop Agomir / Antagomir synthesis services, covering the complete workflow from sequence design, chemical modification, purification, and quality control to final delivery, suitable for both cellular assays and in vivo functional studies.
Figure 1. Chemically modified agomir or antagomir.
Compared to standard miRNA mimics or unmodified antisense oligonucleotides, chemically optimized agomirs and antagomirs offer distinct advantages:
Our agomirs and antagomirs incorporate chemical enhancements to maximize stability, activity, and safety:
| Service Type | Scale | Purification | Purity | Price |
| miR-Down™ Antagomir | 2 OD | HPLC | ≥95% | Inquiry |
| Antagomir Negative Control | 2 OD | HPLC | ≥95% | Inquiry |
| miR-UP™ Agomir | 2 OD | HPLC | ≥95% | Inquiry |
| Agomir Negative Control | 2 OD | HPLC | ≥95% | Inquiry |
Notes:
Each batch undergoes rigorous quality control, including:
In vitro (cell-based) studies
In vivo (animal) studies
Researchers used custom miRNA agomirs and antagomirs synthesized by Creative Biogene to modulate miRNA expression in skeletal muscle. AntagomiR-22-3p and agomiR-127 were applied to enhance myoblast proliferation in vitro and induce muscle hypertrophy in vivo. Treatments effectively regulated target miRNAs and downstream genes such as KIF3B and ACVR2a, leading to distinct proteomic and muscle fiber changes. The study demonstrated the use of synthetic miRNA modulators to influence gene expression and muscle development.
Figure 2. Myoblast proliferation over time in cultures treated with 500 nM antagomiRs (ANT-22-3p, −27a, −29a, and −133). (Greene MA, et al., 2023)
For sequence design, dosing recommendations, or in vivo delivery planning, provide your experimental goal, cell line/animal model, and desired endpoints. Creative Biogene's technical team offers feasible design and optimization guidance to maximize success and efficiency.
Our mission is to provide stable, reproducible, high-quality miRNA modulators. Whether for mechanistic studies, pharmacological validation, or preclinical models, Creative Biogene's Agomir / Antagomir synthesis services deliver technical reliability and efficient support. Contact us for customized solutions and quotations.
Custom miRNA Microarray Service
circRNA-miRNA/RBP Interaction Analysis Service
Q1: Can agomirs be used in vivo without a delivery vector?
A1: Most cholesterol- and PS-modified agomirs/antagomirs can be administered intravenously or intraperitoneally without carriers, but dosage, injection route, and animal model affect efficiency and distribution. Small-scale dose escalation and tissue distribution validation are recommended.
Q2: Can human miRNAs be used directly in mice/rats?
A2: Highly conserved miRNAs may work across species; divergent sequences may fail to bind or produce different phenotypes. Verify sequence conservation and perform in vitro validation prior to in vivo studies.
Q3: How long for agomirs to enter cells without transfection reagents?
A3: Unmodified small RNAs enter cells poorly. Cholesterol and other lipophilic modifications improve passive uptake, but efficiency depends on cell type and modification; transfection reagents or electroporation are recommended for controlled, efficient delivery.
Q4: How to design effective Agomirs or Antagomirs?
A4: Select mature miRNA sequences from authoritative databases like miRBase, considering sequence conservation for cross-species use. Design antagomirs as antisense to the mature miRNA, while agomir guide and passenger strands should mimic the seed region and base-pairing of the endogenous miRNA. Apply chemical modifications such as terminal PS bonds, 3′ cholesterol, and full-length 2′-O-Me to enhance stability and affinity; alternatives like LNA or 2′-F can optimize performance or reduce toxicity. Always include validated positive and negative controls to ensure reproducibility and accurate result interpretation.
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