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Virology

protocols
Protocol

Baculovirus Expression Protocol

Experiment Tags: Baculovirus, Baculovirus expression vector system, BEVS

Experiment Summary: BEVS is recognized as a powerful tool for the expression of the large-scale protein on the strength of convenient process, high expression level and the function of post-translational modifications.

protocols
Protocol

Lentivirus Transduction Protocol

Experiment Tags: Lentivirus, Lentivirus Transduction

Experiment Summary: Lentivirus is a modified HIV virus that must be handled with caution although it cannot replicate in a host.

protocols
Protocol

Adenoviral Vectors Production Protocol

Experiment Tags: Adenoviral Vectors, Adenovirus

Experiment Summary: The adenoviral vector is part of a bacterial plasmid. In order to release the adenoviral ends, it is necessary to digest with Pac I. The best method is to digest 12 µg of the plasmid in 100 µl total volume for 3 hours.

protocols
Protocol

Viral Titering-Plaque Assay Protocol

Experiment Tags: Viral Titering, Plaque Assay

Experiment Summary: After fixing and staining the infected cellular monolayer, plaques are counted so that titer viral stock samples in terms of plaque forming units (pfu) per milliliter.

protocols
Protocol

Viral Titering-TCID50 Assay Protocol

Experiment Tags: Viral Titering, TCID50 Assay

Experiment Summary: A TCID50 was found of greatest value when lack of experimental material or costs of analysis precludes extensive replicate titer determinations but good estimates of titers and treatment differences are essential.