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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NCI-H446 (also known as H446) is a human small cell lung cancer (SCLC) cell line established in 1982 from the pleural effusion of a 61-year-old male patient. SCLC is a highly aggressive form of lung cancer characterized by a short cell doubling time and a strong propensity for early metastasis. NCI-H446 cells are frequently utilized as a classic model for the study of "limited-stage" SCLC. This cell line exhibits neuroendocrine characteristics, including the presence of dense-core granules and the expression of various neuroendocrine markers. It holds immense value in the study of tumor heterogeneity, the investigation of multidrug resistance (MDR) mechanisms, and the development of novel targeted therapies and immunotherapies for small cell lung malignancies. |
| Tissue | Lung; derived from metastatic site (Pleural effusion) |
| Disease | Small Cell Lung Cancer (SCLC) |
| Morphology | Epithelial-like; grows as aggregates or floating clusters |
| Gender | Male |
| Age | 61 years |
| Product Format | Frozen |
| Growth Mode | Adherent and suspension (Mixed) |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Small cell lung cancer (SCLC) pathogenesis and metastasis research 2. Study of neuroendocrine differentiation in lung tumors 3. Investigation of multidrug resistance (MDR) and chemotherapy sensitivity 4. Screening for small-molecule inhibitors and targeted agents 5. Development of SCLC xenograft and PDX-like models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in immunocompromised (nude) mice |
| Karyotype | Aneuploid; highly complex chromosomal rearrangements |
| Genetic Profile | TP53 mutation; RB1 mutation (null status); MYC amplification |
| Neuroendocrine Markers | Positive for Neuron-Specific Enolase (NSE), Chromogranin A, and Synaptophysin |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. For the adherent population: Remove medium, rinse with PBS, and treat with 0.25% Trypsin-EDTA for 3-5 minutes. 2. For the suspension population: Collect the culture medium and centrifuge to harvest cells. 3. Combine both populations if a total culture is desired. 4. Add fresh complete growth medium and gently pipette to break up large aggregates. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00807 | Luciferase Reporter Cell Line - NCI-H446 | Inquiry |
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