NCI-H460 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	NCI-H460, also known as H460, is a cell line originally derived from a male patient diagnosed with large cell lung cancer. These adherent cells exhibit a rapid growth rate. Notably, NCI-H460 cells are able to form tumors in both in vitro and in vivo models, including nude mice. These cells exhibit significant p53 mRNA expression, very similar to levels observed in normal lung tissue, while exhibiting the absence of significant structural DNA abnormalities. These cells stain positively for keratin and vimentin, but negatively for neurofilament triad protein. Isozyme analysis reveals unique expression patterns, such as HPRT located on its surface and varying levels of AK-1, ES-D, Me-2, G6PD, PGM1, and PGM3 isozymes. This cell line has been widely used as a model system to study various aspects of lung cancer biology, including tumor initiation, progression, and drug resistance.
Tissue	Lung
Disease	Lung large cell carcinoma
Morphology	Epithelial
Gender	Male
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug testing and development 3. Genetic studies 4. Molecular pathway studies 5. Radiation research 6. Biomarker discovery 7. Toxicology studies 8. Immunotherapy research
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	These lung cancer cells have many NCI-NCI-H460 mutations similar to those in non-small cell lung tumors, such as NCI-H460 KRAS mutations, which are involved in cell proliferation, growth, invasion, and metastasis.
Characteristics
Karyotype	NCI-H460 cells possess a hypotriploid karyotype with a modal chromosome number of 57, ranging from 53 to 65.
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes given are for 75 cm² flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium required proportionally. 1. Remove and discard the medium. 2. Rinse the cell layer briefly with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 ml of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate the cells by gentle pipetting. 5. Aliquot the appropriate cell suspension into new culture vessels. 6. Incubate the culture at 37°C.
Medium Renewal	Twice per week
Subcultivation Ratio	The ratio of 1:3 to 1:8 is recommended.
Culture Medium	90% RPMI 1640 + 10% h.i. FBS
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37°C
Cryopreservation	Frozen with 70% medium, 20% FBS, 10% DMSO

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Cat.No.	Product Name	Price
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CSC-RR0138	GFP Stable Cell Line-NCI-H460	Inquiry
CSC-RR0380	Luciferase Reporter Cell Line - NCI-H460	Inquiry

* For research use only. Not intended for any clinical use.

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