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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-MEL-1 is a human malignant melanoma cell line isolated in 1968 from the thoracic duct lymph nodes of a 29-year-old Caucasian male with extensive metastatic melanoma. Unlike many other melanoma cell lines that grow in an adherent monolayer, SK-MEL-1 is characterized by suspension growth, often forming large, spherical cell clusters. This cell line is renowned for its high melanin-producing capacity and is frequently used to study melanin production, tumor antigen expression, and the efficacy of immunotherapies. It is an important model for studying the biology of metastatic melanoma and developing targeted therapies for advanced skin cancer. |
| Tissue | Skin; derived from metastatic site (Thoracic duct lymph) |
| Disease | Malignant Melanoma |
| Morphology | Lymphoblast-like; Spherical |
| Gender | Male |
| Age | 29 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Melanoma metastasis and progression research 2. Melanin production and pigmentation regulation research 3. Evaluation of immunotherapy and cancer vaccines 4. Drug screening for metastatic skin cancer 5. Tumor-specific antigen expression research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Pseudodiploid; Modal number = 46 |
| Tumorigenic | Yes, in nude mice |
| Antigen Expression | Blood group AB; Rh+; HLA-A2, A29, B12, Bw35 |
| Specific Markers | High tyrosinase activity; Melanin production |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Cell culture can be maintained by adding fresh culture medium or changing the culture medium. 2. Alternatively, cell culture can be established by centrifugation and resuspending in fresh culture medium. 3. To maintain optimal growth, the cell density should be maintained between 2 × 10⁵ and 1 × 10⁶ viable cells/mL. 4. The cell density should not exceed 1 × 10⁶ cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Culture Medium | Eagle's Minimum Essential Medium (EMEM) or RPMI 1640 supplemented with 10% to 15% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00832 | GFP Reporter Cell Line - SK-MEL-1 | Inquiry |
| CSC-RR00833 | Luciferase Reporter Cell Line - SK-MEL-1 | Inquiry |
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