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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascites of a 44-year-old Caucasian male with primary pancreatic adenocarcinoma that had metastasized to the liver, diaphragm, and lymph nodes. HPAF-II cells exhibit epithelial morphology and are known for their ability to form tumors when xenografted into immunocompromised mice. HPAF-II cells are characterized by specific genetic mutations and alterations that are frequently observed in pancreatic adenocarcinoma. These mutations include mutations in the KRAS gene, which plays a key role in cell signaling and proliferation, and alterations in tumor suppressor genes such as TP53 and CDKN2A. The cell line also exhibits high levels of mucin production, a feature that contributes to the aggressive nature of pancreatic tumors. Researchers frequently use HPAF-II cells to study the molecular mechanisms behind pancreatic cancer progression, including genetic and epigenetic alterations, signal transduction pathways, and interactions with the tumor microenvironment. Studies utilizing HPAF-II cells have provided important insights into the biology of pancreatic cancer and have facilitated the development of potential therapeutic strategies designed to target key molecular pathways implicated in the disease. |
| Tissue | Pancreas |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 44 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug screening and development 3. Metastasis studies 4. Genomic and proteomic studies 5. Tumor microenvironment studies 6. Signal transduction pathways 7. Gene editing and genetic studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Immunofluorescence analysis in HPAF-II cells revealed tight junction localization of ZO-1, occludin, and claudin-4; adherens junction localization of E-cadherin and β-catenin; and desmosomal localization of desmocollin. Transmission electron microscopy showed the presence of tight junctions, adherens junctions, and desmosomes, while freeze-fracture electron microscopy revealed the presence of distinct anastomosing tight junction chains. Transepithelial electrical resistance and permeability measurements showed functional tight junctions. |
| Characteristics | |
| Antigen Expression | Blood Type A; Rh-; HLA A1, A10 (W34), B8, W22, Cw3 |
| Genes Expressed | Mucin |
| Tumorigenic | Yes, these cells formed well-differentiated adenocarcinomas in athymic mice that resembled the original tumors. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Volumes given are for 75 cm2 flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium required proportionally. 1. Remove and discard the medium. 2. Rinse the cell layer briefly with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette out the cells. 5. Add an appropriate aliquot of the cell suspension to a new culture vessel. 6. Incubate the culture at 37°C. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:3 is recommended. |
| Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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|---|---|---|
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