Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HEC-1-B is a human endometrial adenocarcinoma cell line established in 1968 from a 71-year-old patient. It is a subline of the original HEC-1 cell line (belonging to the same lineage as HEC-1-A). Although these two cell lines are homologous, HEC-1-B cells exhibit a more rounded morphology and tend to grow as multilayered cell aggregates rather than in a simple monolayer arrangement. This cell line is widely utilized as an in vitro research model for Type I endometrial carcinoma. Notably, compared to its homologous sister cell line, HEC-1-B demonstrates lower sensitivity to hormonal stimulation. Consequently, it is frequently employed in research areas involving cell cycle regulation, chromosomal abnormalities in uterine cancer, and the screening of chemotherapeutic agents. |
| Tissue | Endometrium; Uterus |
| Disease | Adenocarcinoma; Endometrial Cancer |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | 71 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Endometrial cancer biology and tumorigenesis research 2. Study of chromosomal instability and aneuploidy in cancer cells 3. Drug screening and cytotoxicity assays for gynecological oncology 4. Investigation of protein expression and secretory pathways in uterine cells 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid; Modal number = 47; range = 40 to 48 |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | KRAS mutation; PTEN mutation; TP53 wild-type; MSI (Microsatellite Instability) positive |
| Receptors Expressed | Low to moderate expression of Estrogen Receptor (ER) and Progesterone Receptor (PR) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 5 to 12 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00775 | Luciferase Reporter Cell Line - HEC-1-B | Inquiry |
| CSC-RR00776 | GFP Reporter Cell Line - HEC-1-B | Inquiry |
Copyright © Creative Biogene. All rights reserved.