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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MKN-74 is a human adenocarcinoma cell line derived from a moderately differentiated tubular adenocarcinoma of the stomach in a 37-year-old Japanese male patient. This cell line exhibits an epithelial morphology, grows adherently, and retains many characteristics of intestinal-type gastric cancer. The MKN-74 cell line is notable for its moderately differentiated characteristics and expresses relevant markers such as E-cadherin (CDH1) and CEA. MKN-74 cells harbor a mutation in the TP53 gene—a tumor suppressor gene frequently mutated in gastric cancer. Furthermore, MKN-74 expresses the γ-synuclein (SNCG) gene; studies have demonstrated that the overexpression of SNCG is closely associated with enhanced proliferative capacity and metastatic potential in gastric cancer cells. This cell line is widely utilized to investigate the molecular pathogenesis of intestinal-type gastric cancer, mechanisms of cell-cell adhesion, and the roles of various growth factor receptors in tumor progression. Additionally, it serves as a crucial model for screening chemotherapeutic agents and targeted molecular therapies. |
| Tissue | Stomach; derived from metastatic site (Liver) |
| Disease | Adenocarcinoma; Gastric Cancer (Well-differentiated) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 37 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on well-differentiated gastric adenocarcinoma biology 2. Study of gastric cancer metastasis and liver colonization 3. Evaluation of targeted therapies (e.g., HER2/ERBB2 inhibitors) 4. Investigation of cell adhesion and E-cadherin signaling 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid; modal number in the triploid range |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | TP53 mutation; HER2 (ERBB2) amplification/overexpression; Wild-type E-cadherin (CDH1) |
| Expression Markers | Positive for Carcinoembryonic Antigen (CEA) and epithelial markers |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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