NCI-H520 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The cell line was established in 1982 from a sample of a lung mass taken by A.F. Gazdar from a patient with squamous cell carcimoma of the lung. This cell line expressed greatly reduced levels of p53 mRNA compared with normal lung tissue. These cells showed no obvious structural abnormalities in their DNA. The cells stained positive for keratin and vimentin but negative for neurofilament triad proteins. Cells can form colonies on soft agar with/without serum.
Tissue	Lung
Disease	Lung squamous cell carcinoma
Morphology	Epithelial
Gender	Male
Age	Age unspecified
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Drug screening 2. Genetic studies 3. Oncology research 4. Metabolic studies 5. Protein expression studies 6. Signal transduction studies 7. Tumor growth studies
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The growth inhibitory effect of cuminaldehyde (CuA) on NCI-H520 cells was accompanied by downregulation of proliferation controls involving apoptosis and topoisomerase I and II activity, as well as upregulation of lysosomes, resulting in increased VAC and cytotoxicity. CuA treatment had a protective effect on NCI-H520 xenograft growth in nude mice without any observable toxicity. Therefore, CuA has antiproliferative effects in NCI-H520 cells and has the potential to serve as an antiproliferative agent in cancer.
Characteristics
Karyotype	This is a hypotriploid human cell line. The mode chromosome number is 58, and the incidence is 30%. The frequency of higher ploidies is 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells.
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). 4. NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 5. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gentle pipetting. 6. Add appropriate aliquots of cell suspension to new culture vessels. 7. Cultures were grown at 37°C in the absence of CO₂.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:3 to 1:6 is recommended.
Culture Medium	Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

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* For research use only. Not intended for any clinical use.

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