NIH3T3 Cell Line
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NIH3T3 Cell Line
| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated at the New York University School of Medicine Department of Pathology in 1962. 3T3 refers to the cell transfer and inoculation method for the line, and means “3-day transfer, inoculum 3 x 105 cells.” Using this method, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. George Todaro and Howard Green originally obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. |
| Strain | NIH/Swiss |
| Tissue | Embryo |
| Morphology | Fibroblast |
| Age | Embryo |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | DNA transfection studies NIH 3T3 cell line has been used to:
|
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Not specified |
| Images |  |
| Products | Not specified |
| Receptors | Not specified |
| Mycoplasma | Contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, PCR assays, RNA hybridization |
| Cytogenetics | Murine hypertriploid karyotype with 3% polyploidy - 68(58-73)<3n> |
| Viruses | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Comments | The original cells are extremely contact inhibited, although the cell line is no longer inhibited. NIH 3T3 cells are receptive to transformation with SV40 and polyomavirus. Moreover, 3T3 cells are sensitive to sarcoma virus focus formation, leukemia virus and are inhibited by temazepam and other benzodiazepines. |
| Culture Conditions and Handling | |
| Culture Medium | DMEM + 2 mM Glutamine + 10% Calf Serum (CS). |
| Subculturing |
|
| Split Ratio | Split culture at 1:10 every 4 to 6 days using trypsin/EDTA |
| Medium Renewal | Twice per week |
| Culture Temperature | 37°C |
| Incubation Condition | Carbon dioxide (CO2), 5% |
| Doubling Time | Ca. 20 hours |
| Harvest | Cell harvest of about 20 x 106 cells/175 cm2 flask |
| Storage | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0024 | Human NF-kB Prom/Luciferase/Hyg Reporter Stable Cell Line-NIH3T3 | Inquriy |
| CSC-RR0029 | Human HIF Prom/Luciferase/Hyg Reporter Stable Cell Line-NIH3T3 | Inquriy |
| CSC-RR0057 | GFP Reporter Stable Cell Line-NIH3T3 | Inquriy |
| CSC-RR0300 | IL6-Luc Reporter Cell Line-NIH3T3 | Inquriy |
| CSC-RT0084 | Zdhhc9 Knockout Cell Line-NIH3T3 | Inquriy |
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