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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated at the New York University School of Medicine Department of Pathology in 1962. 3T3 refers to the cell transfer and inoculation method for the line, and means “3-day transfer, inoculum 3 x 105 cells.” Using this method, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. George Todaro and Howard Green originally obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. |
| Strain | NIH/Swiss |
| Tissue | Embryo |
| Morphology | Fibroblast |
| Age | Embryo |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Applications | DNA transfection studies NIH 3T3 cell line has been used to:
|
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Culture Conditions and Handling | |
| Subculturing |
|
| Split Ratio | Split culture at 1:10 every 4 to 6 days using trypsin/EDTA |
| Medium Renewal | Twice per week |
| Culture Temperature | 37°C |
| Incubation Condition | Carbon dioxide (CO2), 5% |
| Doubling Time | Ca. 20 hours |
| Harvest | Cell harvest of about 20 x 106 cells/175 cm2 flask |
| Storage | Frozen with 70% medium, 20% FBS, 10% DMSO |
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