Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | In 1893, Dan L. Longo and Walter J. Urba established the RL cell line from the ascites of a 52-year-old Caucasian male diagnosed with non-Hodgkin lymphoma (NHL) affecting B cells. Since then, RL cells have been used as a model for studying B-cell NHL. The RL cell line can be used to create the CDX (cell line derived xenograft) RL xenograft mouse model. The RL xenograft model has been used as a model for studying non-Hodgkin lymphoma treatments (immunotherapy using antibody conjugates, etc.). |
| Tissue | Ascites |
| Disease | Lymphoma; Non-Hodgkin's |
| Morphology | Lymphoblast |
| Gender | Male |
| Age | 52 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Immunology studies 3. Drug screening and development 4. Gene expression analysis |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Exposure of RL cells to phorbol esters (such as PMA and PdBu) that activate protein kinase C results in severe growth inhibition. RL cells are negative for the Epstein-Barr virus genome. |
| Characteristics | |
| Karyotype | The cells possess a 14:18 translocation |
| Gene Expressed | Immunoglobulin, surface IgM; and IgD; secreted IgM (low levels) |
| Antigen Expression | CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - |
| Tumorigenic | Yes |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Cultures can be maintained by adding or replacing media. Establish new cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 and 1 x 106. |
| Medium Renewal | 2 to 3 times a week. |
| Culture Medium | 90% RPMI 1640 + 10% Foetal Bovine Serum (FBS) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0590 | GFP Reporter Cell Line - RL | Inquiry |
| CSC-RR0591 | Luciferase Reporter Cell Line - RL | Inquiry |
| CSC-RR0592 | GFP/Luc Reporter Cell Line - RL | Inquiry |
Copyright © Creative Biogene. All rights reserved.