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CHO-K1 Cell Line

General Information
OrganismCricetulus griseus (hamster, Chinese)
Cell Line DescriptionA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They experience morphological changes in response to cholera toxin.
Tissue of OriginOvary
Cell TypeEpithelial
Product FormatFrozen
Growth ModeAdherent
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Application(s)Cell culture | mammalian: suitable
Shipped inDry ice
Storage Temperature−196°C
ReferencesJ Exp Med 1958;108:945Proc Nat Acad Sci USA 1968;60:1275
KaryotypeHypodiploid, modal no. 20
ImagesCHO-K1 Cell Line
Clinical DataFemale
VirusesSMRV: Negative, as confirmed by Real-Time PCR
Virus SusceptibilityVesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Getah virus
Virus ResistanceModoc virus; poliovirus 2; Button Willow virus
Culture Conditions and Handling
Culture MediumTo make the complete growth medium, add the following components to a base medium: fetal bovine serum to a final concentration of 10%.

Volumes are given for a 75 cm2 flask. Decrease or increase the amount of dissociation medium needed proportionally for culture vessels of other sizes.

  1. Remove and discard the culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5-15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal.
  4. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Split Ratio1x104 cells/cm2 will yield in a confluent layer in about 6 days
Fluid RenewalEvery 2-3 days
Doubling TimeAbout 22 h
Freeze MediumComplete growth medium 95%; DMSO, 5%
Freezing RecoveryAllow the cells to recover from the freezing process for at least 24 h
Culture Temperature37°C
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