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CHO-K1 Cell Line


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CHO-K1 Cell Line

General Information
OrganismCricetulus griseus (hamster, Chinese)
Cell Line DescriptionA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They experience morphological changes in response to cholera toxin.
Tissue of OriginOvary
Cell TypeEpithelial
Product FormatFrozen
Growth ModeAdherent
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Application(s)Cell culture | mammalian: suitable
Shipped inDry ice
Storage Temperature−196°C
ReferencesJ Exp Med 1958;108:945Proc Nat Acad Sci USA 1968;60:1275
Characteristics
KaryotypeHypodiploid, modal no. 20
ImagesCHO-K1 Cell Line
Clinical DataFemale
VirusesSMRV: Negative, as confirmed by Real-Time PCR
Virus SusceptibilityVesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Getah virus
Virus ResistanceModoc virus; poliovirus 2; Button Willow virus
Culture Conditions and Handling
Culture MediumTo make the complete growth medium, add the following components to a base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes are given for a 75 cm2 flask. Decrease or increase the amount of dissociation medium needed proportionally for culture vessels of other sizes.

  1. Remove and discard the culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5-15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal.
  4. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Split Ratio1x104 cells/cm2 will yield in a confluent layer in about 6 days
Fluid RenewalEvery 2-3 days
Doubling TimeAbout 22 h
Freeze MediumComplete growth medium 95%; DMSO, 5%
Freezing RecoveryAllow the cells to recover from the freezing process for at least 24 h
Culture Temperature37°C
Cat.No. Product Name Price
CSC-RG0253Mouse Ffar3-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0255Human FFAR3-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0259Human GPR84-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0261Human GALR1-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0289Human S1PR1-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0291Human S1PR3-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0292Human S1PR4-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0293Human S1PR5-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0331Human NPY1R-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0332Human NPY2R-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0333Human PPYR1-FLAG Stable Cell Line-CHO-K1 Galphaqi5Inquriy
CSC-RG0396Human SSTR1-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0398Human SSTR2-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0400Human SSTR3-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0402Human SSTR4-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0404Human SSTR5-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG0438Human FFAR2-FLAG Stable Cell Line-CHO-K1 Galpha16Inquriy
CSC-RG1642Human HTR1A Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1643Human CHRM4 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1644Human ADORA2A Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1645Human ADORA2B Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1646Human ADRA1D Stable Cell Line-CHO-K1Inquriy
CSC-RG1647Human ADRA2B Stable Cell Line-CHO-K1/Gqi5Inquriy
CSC-RG1648Human ADRA2C Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1649Human APLNR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1650Human GPBAR1 Stable Cell Line-CHO-K1Inquriy
CSC-RG1651Human NMBR Stable Cell Line-CHO-K1Inquriy
CSC-RG1652Human BRS3 Stable Cell Line-CHO-K1Inquriy
CSC-RG1653Human BDKRB2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1654Human CALCR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1655Human CASR Stable Cell Line-CHO-K1Inquriy
CSC-RG1656Human CNR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1657Human CMKLR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1658Human CCR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1659Human CCR5 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1699Human QRFPR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1700Human PTAFR Stable Cell Line-CHO-K1Inquriy
CSC-RG1701Human PTGDR2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1702Human PTGIR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1703Human PTGDR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1704Human F2RL1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1705Human SSTR1 Stable Cell Line-CHO-K1/Gqi5Inquriy
CSC-RG1706Human SSTR2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1707Human SSTR3 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1708Human SSTR4 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1709Human SSTR5 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1710Human TACR1 Stable Cell Line-CHO-K1Inquriy
CSC-RG1711Human UTS2R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1712Human OXTR Stable Cell Line-CHO-K1Inquriy
CSC-RG1713Human VIPR2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1714Human VIPR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1729Rat EDNRA Stable Cell Line-CHO-K1Inquriy
CSC-RG1730Human EDNRB Stable Cell Line-CHO-K1Inquriy
CSC-RG1735Mouse Hrh3 Stable Cell Line-CHO-K1Inquriy
CSC-RG1737Mouse GRM4 Stable Cell Line-CHO-K1 Gαqi5Inquriy
CSC-RG1738Rat Npy2r Stable Cell Line-CHO-K1Inquriy
CSC-RG1739Human NTSR1 Stable Cell Line-CHO-K1Inquriy
CSC-RG1743Mouse Gpr183 Stable Cell Line-CHO-K1Inquriy
CSC-RG1748Human CXCR4 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1749Human DRD2(S) Stable Cell Line - CHO-K1-Gα15Inquriy
CSC-RG1750Human HTR4 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1751Human CHRM2 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1752Human ADORA1 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1754Mouse Adora2a Stable Cell Line - CHO-K1Inquriy
CSC-RG1755Human ADORA3 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1756Human ADRA2A Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1757Human ADRB1 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1758Human RAMP1+CALCR Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1759Human RAMP2+CALCR Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1760Human RAMP3+CALCR Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1761Human CCKAR Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1762Human CRHR2 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1763Human FFAR1 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1764Human GALR1 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1766Human HRH4 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1767Human CYSLTR1 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1768Human LTB4R Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1769Human MC4R Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1770Human MTNR1B Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1772Human PTGER2 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1773Human AVPR2 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1774Human ADCYAP1R1 Stable Cell Line - CHO-K1/Gα15Inquriy
CSC-RG1777Human MCHR2 Stable Cell Line - CHO-K1Inquriy
CSC-RR0088tGFP Reporter Stable Cell Line-CHO-K1Inquriy
CSC-RG1660Human CXCR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1661Human CXCR3 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1662Human CXCR5 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1663Human GPR68 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1664Human MRGPRX2 Stable Cell Line-CHO-K1Inquriy
CSC-RG1665Human C5AR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1666Human CRHR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1667Human DRD2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1668Human DRD5 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1669Human DRD1 Stable Cell Line-CHO-K1Inquriy
CSC-RG1670Human FPR1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1671Human FFAR2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1672Human GLP2R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1673Human GCGR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1674Human SCTR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1675Human GIPR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1676Human GNRHR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1677Human GHSR Stable Cell Line-CHO-K1Inquriy
CSC-RG1678Human HRH2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1679Human HRH3 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1680Human HCAR2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1681Human KISS1R Stable Cell Line-CHO-K1Inquriy
CSC-RG1682Human FPR2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1683Human MC2R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1684Human MC3R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1685Human MC5R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1686Human MTNR1A Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1687Human GRM2 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1688Human MLNR Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1689Human NMUR1 Stable Cell Line-CHO-K1Inquriy
CSC-RG1690Human NPSR1 (b) Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1691Human NPSR1 (a) Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1692Human NPY2R Stable Cell Line-CHO-K1/Gqi5Inquriy
CSC-RG1693Human NPY4R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1694Human OPRD1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1695Human OPRK1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1696Human OPRM1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RG1697Human OPRL1 Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RR0095FP602 Reporter Stable Cell Line-CHO-K1Inquriy
CSC-RR0403TCF/LEF Luciferase Reporter Cell Line - CHO-K1Inquriy
CSC-RR0405ATF6 Luciferase Reporter Cell Line - CHO-K1Inquriy
CSC-RO0190Monkey TNFRSF9 Stable Cell Line - CHO-K1Inquriy
CSC-RO0191Human CLEC4C Stable Cell Line - CHO-K1Inquriy
CSC-RO0192Monkey CLEC4C Stable Cell Line - CHO-K1Inquriy
CSC-RO0193Human CLEC4C + FCER1G Stable Cell Line - CHO-K1Inquriy
CSC-RO0194Monkey CLEC4C + FCER1G Stable Cell Line - CHO-K1Inquriy
CSC-RO0195Human PVR Stable Cell Line - CHO-K1Inquriy
CSC-RO0196Monkey PVR Stable Cell Line - CHO-K1Inquriy
CSC-RO0197Mouse Pvr Stable Cell Line - CHO-K1Inquriy
CSC-RO0198Mouse Cd38 Stable Cell Line - CHO-K1Inquriy
CSC-RO0199Mouse Cd47 Stable Cell Line - CHO-K1Inquriy
CSC-RO0200Monkey CD47 Stable Cell Line - CHO-K1Inquriy
CSC-RO0201Human NECTIN2 Stable Cell Line - CHO-K1Inquriy
CSC-RO0202Monkey CTLA4 Stable Cell Line - CHO-K1Inquriy
CSC-RO0203Human FLT3 Stable Cell Line - CHO-K1Inquriy
CSC-RO0204Monkey FLT3 Stable Cell Line - CHO-K1Inquriy
CSC-RO0205Human TNFRSF14 Stable Cell Line - CHO-K1Inquriy
CSC-RO0206Mouse Tnfrsf14 Stable Cell Line - CHO-K1Inquriy
CSC-RO0207Human KIR2DL1 Stable Cell Line - CHO-K1Inquriy
CSC-RO0208Monkey PDCD1 Stable Cell Line - CHO-K1Inquriy
CSC-RO0210Monkey CD274 Stable Cell Line - CHO-K1Inquriy
CSC-RO0211Mouse Pdcd1lg2 Stable Cell Line - CHO-K1Inquriy
CSC-RO0212Monkey PDCD1LG2 Stable Cell Line - CHO-K1Inquriy
CSC-RO0213Mouse Vsir Stable Cell Line - CHO-K1Inquriy
CSC-RO0214Monkey VSIR Stable Cell Line - CHO-K1Inquriy
CSC-RO0215Monkey TIGIT Stable Cell Line - CHO-K1Inquriy
CSC-RO0227Human ITGAE Stable Cell Line - CHO-K1Inquriy
CSC-RO0228Monkey HAVCR2 Stable Cell Line - CHO-K1Inquriy
CSC-RG1698Human PTH1R Stable Cell Line-CHO-K1/Gα15Inquriy
CSC-RT0097GS/Fut8 Knockout Cell Line-CHO-K1Inquriy
CSC-RT0103GS/Bax/Bak1 Knockout Cell Line-CHO-K1Inquriy
CSC-RT0119GS/BAX Knockout Cell Line-CHO-K1Inquriy
CSC-RT0125GS Knockout Cell Line-CHO-K1Inquriy
CSC-RO0250TNFRSF17 Stable Cell Line - CHO-K1Inquriy
CSC-RO0251CD19 Stable Cell Line - CHO-K1Inquriy

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