CHO-K1 Cell Line

Brief Introdution
Product List

General Information
Organism	Cricetulus griseus (hamster, Chinese)
Cell Line Description	A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They experience morphological changes in response to cholera toxin.
Tissue of Origin	Ovary
Cell Type	Epithelial
Product Format	Frozen
Growth Mode	Adherent
Application(s)	Cell culture \| mammalian: suitable
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Subculturing	Volumes are given for a 75 cm² flask. Decrease or increase the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard the culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5-15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Split Ratio	1x10⁴ cells/cm² will yield in a confluent layer in about 6 days
Fluid Renewal	Every 2-3 days
Doubling Time	About 22 h
Freeze Medium	Complete growth medium 95%; DMSO, 5%
Freezing Recovery	Allow the cells to recover from the freezing process for at least 24 h
Culture Temperature	37°C

The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-BS0001	Rituximab Stable Cell Line - CHO-K1	Inquiry
CSC-BS0002	Trastuzumab Stable Cell Line - CHO-K1	Inquiry
CSC-BS0003	Cetuximab Stable Cell Line - CHO-K1	Inquiry
CSC-BS0004	Nivolumab Stable Cell Line - CHO-K1	Inquiry
CSC-BS0005	Pembrolizumab Stable Cell Line - CHO-K1	Inquiry
CSC-BS0006	Adalimumab Stable Cell Line - CHO-K1	Inquiry
CSC-BS0007	Infliximab Stable Cell Line - CHO-K1	Inquiry
CSC-RG0023	Human CNR2-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0024	Human CHRM1-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0025	Human ADRB2-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0026	Human BDKRB2-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0028	Human BDKRB1-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0029	Human CRHR2-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0031	Human CALCR-tGFP Stable Cell Line-CHO-K1	Inquiry
CSC-RG0058	Human ADRB2 Stable Cell Line-CHO-K1	Inquiry

* For research use only. Not intended for any clinical use.

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