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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The J82 cell line, derived from a 58-year-old Caucasian male patient with high-grade (T2 stage) transitional cell carcinoma of the bladder, is one of the most widely used models for studying the biology of bladder cancer. This cell line is characterized by its ability to grow as an adherent monolayer with highly rearranged karyotypes. J82 cells are known to express a variety of tumor-associated antigens and are frequently used to study the mechanisms of bladder cancer progression, chemotherapy resistance, and the development of targeted therapies. |
| Tissue | Urinary Bladder |
| Disease | Transitional Cell Carcinoma (Urothelial Carcinoma) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 58 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Bladder cancer research and oncology studies 2. Drug screening and cytotoxicity assays 3. Gene expression and regulation studies 4. Investigation of invasion and metastatic pathways 5. Evaluation of novel therapeutic markers |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Hypertriploid/Polyploid; complex chromosomal rearrangements; Modal number = 66-80 |
| Tumorigenic | Yes, in immunocompromised mice |
| Receptors Expressed | Epidermal Growth Factor Receptor (EGFR) |
| Protein Expression | p53 mutant; Retinoblastoma (Rb) protein deficient |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with Ca²⁺/Mg²⁺-free PBS or 0.25% trypsin-EDTA solution to remove residual serum. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution and observe the cells under a microscope until the cell layer disperses (5 to 15 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Culture Medium | Eagle's Minimum Essential Medium (EMEM) supplemented with 10% Fetal Bovine Serum (FBS) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00784 | Luciferase Reporter Cell Line - J82 | Inquiry |
| CSC-RR00785 | GFP Reporter Cell Line - J82 | Inquiry |
| CSC-RR01042 | mScarlet/LUC Reporter Cell Line - J82 | Inquiry |
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