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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | MBT-2 (Mouse Bladder Tumor-2) is a murine transitional cell carcinoma cell line originally induced in C3H/He mice using the carcinogen N-[4-(5-nitro-2-furanyl)-2-thiazolyl]formamide (FANFT). It is currently the most widely utilized syngeneic, immunocompetent model in bladder cancer research. MBT-2 cells exhibit an epithelial morphology and demonstrate robust tumorigenicity when implanted subcutaneously or orthotopically (i.e., into the bladder wall) in syngeneic C3H mice. This cell line serves as a cornerstone for studies investigating the efficacy of intravesical BCG therapy, evaluating novel immunotherapies (such as immune checkpoint inhibitors), and examining the tumor immune microenvironment within a system that closely recapitulates the progression of human bladder cancer. |
| Tissue | Urinary Bladder |
| Disease | Transitional Cell Carcinoma (TCC); Bladder Cancer |
| Morphology | Epithelial-like |
| Gender | Not specified (derived from C3H/He mouse) |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Bladder cancer pathogenesis and syngeneic model research 2. Evaluation of Intravesical BCG and other immunotherapy protocols 3. Study of tumor-infiltrating lymphocytes (TILs) and immune evasion 4. Drug screening for urothelial carcinoma in an immunocompetent host 5. Development of orthotopic bladder cancer models for preclinical studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in syngeneic C3H/He mice |
| Antigenicity | Exhibits specific tumor-associated antigens; responsive to immune modulation |
| Growth Kinetics | Rapid doubling time in vitro and fast solid tumor formation in vivo |
| Phenotype | Maintains characteristics of high-grade transitional cell carcinoma |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe continuously until the cell monolayer has dispersed (typically requiring 3 to 8 minutes). 4. Add complete growth medium to neutralize the trypsin, and gently pipette to create a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00796 | Luciferase Reporter Cell Line - MBT-2 | Inquiry |
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