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4T1 Cell Line

General Information
OrganismMus musculus, mouse
Cell Line Description4T1 is a murine mammary carcinoma cell line from a BALB/cfC3H mouse. The cells are adherent and have an epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer.
Tissue of OriginMammary gland
Sex of CellFemale
Cell TypeEpithelial
Product FormatFrozen
Growth ModeAdherent
DiseaseThis tumor is an animal stage IV human breast cancer.
Application(s)Cell Culture / Growth Conditions
Shipped inDry ice
Storage Temperature−196°C
Culture Conditions and Handling
SubculturingNote: the cells should not be allowed to become confluent, subculture at 80% of confluence.
  1. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution.
  2. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution.
  3. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
  4. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation RatioA ratio of 1:6 to 1:8 is recommended.
Fluid RenewalEvery 2-3 days
CryopreservationFreeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture ConditionsAtmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C

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* For research use only. Not intended for any clinical use.
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