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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | 4T1 is a murine mammary carcinoma cell line from a BALB/cfC3H mouse. The cells are adherent and have an epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer. |
| Tissue of Origin | Mammary gland |
| Sex of Cell | Female |
| Cell Type | Epithelial |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Disease | This tumor is an animal stage IV human breast cancer. |
| Application(s) | Cell Culture / Growth Conditions |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Culture Conditions and Handling | |
| Subculturing | Note: the cells should not be allowed to become confluent, subculture at 80% of confluence.
|
| Subcultivation Ratio | A ratio of 1:6 to 1:8 is recommended. |
| Fluid Renewal | Every 2-3 days |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
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