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4T1 Cell Line

General Information
OrganismMus musculus, mouse
Cell Line Description4T1 is a murine mammary carcinoma cell line from a BALB/cfC3H mouse. The cells are adherent and have an epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer.
Tissue of OriginMammary gland
Sex of CellFemale
Cell TypeEpithelial
Product FormatFrozen
Growth ModeAdherent
DiseaseThis tumor is an animal stage IV human breast cancer.
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Application(s)Cell Culture / Growth Conditions
Shipped inDry ice
Storage Temperature−196°C
Derivation4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.
Images4T1 Cell Line4T1 cells are transfected with GFP vector (pEGFP-N3). The cells are visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
EffectsYes, forms tumors and metastasizes in BALB/c mice
SterilityBacteria: Negative
Yeast: Negative
Mycoplasma: Negative
PathogensHIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
CommentsWhen injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ. The primary tumor does not have to be removed to induce metastatic growth. The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.
Culture Conditions and Handling
Culture MediumTo make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
SubculturingNote: the cells should not be allowed to become confluent, subculture at 80% of confluence.
  1. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution.
  2. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution.
  3. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
  4. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation RatioA ratio of 1:6 to 1:8 is recommended.
Fluid RenewalEvery 2-3 days
CryopreservationFreeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture ConditionsAtmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C

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For research use only. Not intended for any clinical use.

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