ACHN Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	ACHN is a cell line with epithelial morphology that was isolated in 1979 from the kidney of a 22-year-old Caucasian male patient with adenocarcinoma (renal cell). ACHN cells are characterized by an adherent growth pattern and express specific isoenzymes, including G6PD (type B). The ACHN cell line is tumorigenic, being able to induce tumors in 100% (5/5) of nude mice inoculated with 10⁷ cells, with tumors established within 21 days. The ACHN cell line is a valuable tool in cancer research, especially in the context of renal adenocarcinoma. It is an important model for studying tumorigenicity, metastatic behavior, and the effects of interferons on cancer cell proliferation. Both primary ACHN cells and cells recovered from nude mouse tumors show growth inhibition in the presence of human interferons, highlighting their potential application in studies exploring the efficacy of interferon-based therapies for renal cancer.
Tissue	Kidney
Disease	Adenocarcinoma; Renal Cell
Morphology	Epithelial
Gender	Male
Age	22 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug screening and development 3. Gene expression studies 4. Cell signaling pathway analysis 5. 3D cell culture 6. In vivo studies
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The ACHN cell line has a c-met polymorphism that is specific to papillary renal cell carcinoma (RCC). Chromosomal aberrations in ACHN cells also resemble those in papillary tumors. However, gene expression analysis revealed similarities to clear cell tumors, especially when involving the MYC pathway. No mutations in VHL and HIF-1α mRNA in ACHN cell line, could confirm non-clear cell histology.
Characteristics
Tumorigenic	Yes, in nude mice
Receptors Expressed	CAIx- (carbonic anhydrase Ix)
Protein Expression	p53 positive
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes given are for 75 cm2 flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium required proportionally. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin - 0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently remove the cells by pipetting. 5. Add an appropriate amount of the cell suspension to a new culture vessel. 6. Incubate the culture at 37℃.
Medium Renewal	2 to 3 times per week.
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended.
Culture Medium	EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01162	Cas9 Stable Cell Line - ACHN	Inquiry
CSC-RR00632	GFP Reporter Cell Line - ACHN	Inquiry
CSC-RR00659	Luciferase Reporter Cell Line - ACHN	Inquiry
CSC-RR01032	mScarlet/LUC Reporter Cell Line - ACHN	Inquiry

* For research use only. Not intended for any clinical use.

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