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Immune receptors are protein molecules, usually expressed on the membrane of immune related cells. These receptors can interact with their corresponding ligands to further regulate immune responses. Immune checkpoints and toll-like receptors are important molecules in the immune system. The main receptors in the immune system are pattern recognition receptors (PRR), Toll-like receptors (TLRs), killer activating and killer inhibitory receptors (KAR and KIR), complement receptors, Fc receptors, B cell receptors and T cell receptor.
Immune receptor stable cell lines are an essential tool in immunological research and drug discovery. These engineered cell lines have permanently integrated a single type of immune receptor gene into their genome, enabling persistent and homogeneous expression of the immune receptor protein on their surfaces. Scientists of Creative Biogene have developed a series of immune receptor stable cell lines to assist your research.
Advantages of Our Immune Receptor Stable Cell Lines:
Immunoreceptor stable cell lines are commonly used in a variety of biological, medical, and pharmaceutical research due to their ability to consistently express immune receptors of interest. Here are a few key applications.
Drug discovery: These cell lines are useful in screening new drugs. By targeting specific immune receptors, researchers can analyze the interactions and effectiveness of potential therapeutics. Assessing how drugs interact with their immune receptors can provide valuable insights and help develop more effective, targeted treatments.
Disease research: Immune receptor stable cell lines can be used for research on various diseases, especially immune diseases and cancer. Observing these cell lines can effectively understand the role of immune receptors in disease pathogenesis and thus better understand disease mechanisms.
Immunobiological research: Immunoreceptor stable cell lines are invaluable tools in immunological research. They provide a simplified system for studying complex interactions between immune cells and pathogens, or between immune cells themselves. This can help understand how the immune system responds.
Protein expression and affinity analysis: These cell lines are used for consistent expression of immune receptors. The expressed receptors can then be used for a variety of purposes, such as the study of receptor signaling pathways, receptor-ligand interaction analysis, and affinity determination.
Vaccine development: Using these cell lines to study immune responses can help develop novel vaccines. They elicit specific immune responses and therefore can be used to assess the efficacy of vaccine candidates.
Therefore, immune receptor stable cell lines are powerful tools for driving medical advances in targeted therapies, vaccine development, and disease research.
Case Study 1
Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) has been studied to be involved in the development and progression of a variety of human malignancies. However, little is currently known about the complex mechanisms of TNFRSF11B in human gastric cancer (GC). Here, we demonstrate that TNFRSF11B is highly expressed in the GC cytoplasm and is associated with poor patient prognosis. Studies have shown that TNFRSF11B in GC cells significantly promotes cell proliferation, migration, invasion in vitro, and tumorigenic capacity in vitro and in vivo. Meanwhile, TNFRSF11B inhibited GC cell apoptosis. TNFRSF11B directly binds to GSK-3β to upregulate its phosphorylation and increase the expression of β-catenin and its downstream effectors. Collectively, these findings indicate that TNFRSF11B promotes the invasive phenotype of GC cells and activates Wnt/β-catenin signaling. Therefore, TNFRSF11B has the potential as a biomarker, and inhibition of TNFRSF11B expression may provide a new therapeutic target for GC patients.
Figure 1. Effect of TNFRSF11B on GC cell apoptosis. (A and B) TNFRSF11B overexpressing cell group shows reduced apoptosis rate in HGC-27 and BGC-823 cells. (C and D) TNFRSF11B knockdown cell group shows increased apoptosis rate of MGC-803 and SGC7901 cells. (E) In the TUNEL experiment, the cell apoptosis rate in the HGC-27 TNFRSF11B overexpression group was significantly lower than that in the control group. (F) The cell apoptosis rate in the MGC-803 TNFRSF11B knockdown group was significantly higher than that in the control group. (G) BCL-2 expression is higher and BAX, PARP, and Caspase3 are lower in HGC-27 and BGC-823 TNFRSF11B-overexpressing cells compared with control cells. (H) Low expression of BCL-2 and high expression of BAX, PARP and Caspase3 in MGC-803 and SGC-7901 cells. (Luan F, et al., 2020)
Case Study 2
CD150 (IPO3/SLAM) belongs to the SLAM receptor family and is the primary entry receptor for measles virus. CD150 is expressed on normal and malignant cells of the immune system. Here, researchers show that CD150 is expressed in CNS tumors and glioma cell lines. This antigen was not expressed on the surface of the glioma cells tested but was found in the cytoplasm, colocalizing with the endoplasmic reticulum and Golgi complex. In addition to the traditional splicing isoforms, a novel CD150 isoform (nCD150) with an 83 bp insert was identified. Similar to mCD150, cell surface expression of nCD150 allows wild-type measles virus to enter cells.
Figure 2. Cell lines transfected with mCD150 and nCD150 are susceptible to wild-type measles virus infection. (A) Surface expression of mCD150 and nCD150 isoforms in HEK293T and U87 cell lines after transfection with the corresponding plasmids measured by flow cytometry after staining with anti-CD150 mAb IPO3. (B) Production of wild-type MV (G954 strain) in HEK293T cells transfected with mCD150 and nCD150, measured daily by plaque assay on Vero-SLAM cells, 24 h (1), 48 h (2), 72 hours (3), 96 hours (4) and 120 hours post-infection (5). (Romanets-Korbut O, et al., 2015)
Q: What are Immune Receptor Stable Cell Lines?
A: Immune Receptor Stable Cell Lines are designed to support and enhance immunological research. They are cells that have been genetically modified to express specific immune receptors. This allows for consistent reproduction and study of specific receptors and their functions.
Q: Why are Immune Receptor Stable Cell Lines important in immunology research?
A: They are valuable tools in immunological research because they aid in understanding the function of expressed receptors, their interactions with specific antigens or drugs, and in studying possible mechanisms of immune diseases or in the development of treatments.
Q: What are the advantages of using Creative Biogene's Immune Receptor Stable Cell Lines?
A: The major advantage of Creative Biogene's cell lines is that they provide a consistent, reproducible source of cells expressing the receptor of interest. This could greatly facilitate research and reduce the need for animal models. They are widely used in drug discovery, assay development, and disease modeling.
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