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PCR Series

protocols
Protocol

Standard PCR Protocol

Experiment Tags: PCR, Standard PCR

Experiment Summary: Taq polymerase is frequently used in the polymerase chain reaction (PCR), for it is able to withstand repeated heating to 95 °C without significant loss of activity, an efficient method for greatly amplifying the quantity of DNA templates.

protocols
Protocol

RT-PCR Protocol

Experiment Tags: RT-PCR, PCR

Experiment Summary: RNA is first reverse transcribed into cDNA using a reverse transcriptase as described here, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes.

protocols
Protocol

PCR-ELISA Protocol

Experiment Tags: PCR Amplification, Probe Hybridization, ELISA Assay

Experiment Summary: This experiment describes the procedure of PCR-ELISA experiment based on immunological method to detect PCR products.

protocols
Protocol

PCR Amplification Preparation with Labeled Probes Protocol

Experiment Tags: PCR Amplification, Probe Preparation

Experiment Summary: This experiment introduces the PCR labeling technique of probes using biotin and digoxin as examples.

protocols
Protocol

Protocol for Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR

Experiment Tags: Subcellular Fractionation, RT-qPCR, Circular RNA, circRNA, Cytoplasmic circRNA, Nuclear circRNA, Low-Abundance circRNA

Experiment Summary: In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using a lentiviral expression system.