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Experiment Tags: Subcellular Fractionation, RT-qPCR, Circular RNA, circRNA, Cytoplasmic circRNA, Nuclear circRNA, Low-Abundance circRNA
Experiment Summary: In this protocol, we present a protocol for the subcellular fractionation of cells to detect circRNA via RT-qPCR with divergent primers. Moreover, detailed steps for the generation of specific circRNAs-enriched cDNA included in this protocol will enhance the amplification and detection of low-abundance circRNAs.
Experiment Tags: PCR Amplification, Probe Preparation
Experiment Summary: This experiment introduces the PCR labeling technique of probes using biotin and digoxin as examples.
Experiment Tags: PCR Amplification, Probe Hybridization, ELISA Assay
Experiment Summary: This experiment describes the procedure of PCR-ELISA experiment based on immunological method to detect PCR products.
Experiment Tags: PCR, Standard PCR
Experiment Summary: Taq polymerase is frequently used in the polymerase chain reaction (PCR), for it is able to withstand repeated heating to 95 °C without significant loss of activity, an efficient method for greatly amplifying the quantity of DNA templates.
Experiment Tags: RT-PCR, PCR
Experiment Summary: RNA is first reverse transcribed into cDNA using a reverse transcriptase as described here, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes.
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