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Reporter genes, like GFP or Luciferase, are vital tools in gene expression studies. They are attached to a gene of interest's regulatory sequence for visual identification in cell culture or organisms. Our purified reporter lentiviruses offer single or dual reporter vectors with fluorescent (eGFP, mCherry) and bioluminescent (Firefly, Renilla) markers. These lentiviruses provide a sensitive, dynamic, and straightforward method for promoter activity measurement, gene regulation studies, and drug screening.
Developed by Creative Biogene, our comprehensive line of reporter lentiviruses, expressing luminescent or fluorescent markers, supports diverse research applications, offering ready-to-use particles for efficient gene delivery into various cell types. Explore our product list for the right reporter lentivirus for your needs!
Advantages of Our Reporter Lentiviral Particles
Reporter Lentiviral Particles Product List
The reporter lentiviruses enable real-time tracking of promoter activity and gene expression by stable genomic integration of fluorescent/luminescent cassettes. Quantifying reporter signals provides an accurate readout of gene delivery efficiency and expression kinetics. The principle involves cloning promoter-reporter fusions enabling signal quantification upon lentiviral transduction. Wide cell specificity allows diverse applications from monitoring transfection conditions to studying gene regulation. Creative Biogene's customizable reporter lentiviruses can assist you in the following tasks:
Case Study 1
SARS-CoV-2 utilizes human ACE2 (hACE2) for entry, with mouse ACE2 (mACE2) ineffective. The researchers presented an ACE2-lentivirus system for in vitro and in vivo SARS-CoV-2 infection models. The researcher cloned the hACE2 coding sequence, optimized for mouse codon usage, into the pCDH lentiviral vector with a dual promoter system (EF1α-MCS-BGH-PGK-GFP-T2A-Puro). The EF1α promoter, known for efficient gene expression, was utilized. The vector includes a GFP marker and puromycin resistance gene, expressed from a distinct PGK promoter, with the ability to drive gene expression in mouse lungs after VSV-G pseudotyped lentivirus transduction.
Figure 1. Lentivirus-mediated ACE2 expression in HEK293T cells facilitated SARS-CoV-2 infection studies, assessing growth kinetics, morphology, and viral binding dynamics. (Rawle DJ, et al., 2021)
Case Study 2
Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Researchers achieved a high success rate in generating transgenic rats by in vivo manipulation of spermatogonial stem cells (SSCs) using lentiviral transduction. Juvenile rats were transduced with lentivirus, resulting in founder rats with a 50.0% success rate in passing on the transgene to subsequent generations. This approach proves effective for generating transgenic rats through male germline modification and has the potential for adaptation to larger animal species where existing gene modification methods may be insufficient.
Figure 2. Lentiviral transduction of rat spermatogonial stem cells involved microinjection of eGFP-expressing lentivirus into seminiferous tubules. Fluorescence microscopy revealed green fluorescent tubules as colonies from transduced spermatogonial stem cells. (Kim BJ, et al., 2019)
Q: What is the primary purpose of Reporter Lentiviral Particles in molecular research?
A: Reporter Lentiviral Particles are designed to introduce reporter genes into target cells, enabling real-time monitoring and analysis of gene expression, cellular processes, and experimental outcomes.
Q: How are these lentiviral particles engineered to incorporate reporter genes into cells?
A: Reporter Lentiviral Particles are engineered to carry reporter genes, such as fluorescent proteins or luciferase, which are expressed upon transduction into target cells, providing a visual or quantitative readout.
Q: In what experimental scenarios are Reporter Lentiviral Particles particularly valuable?
A: These particles are valuable in experiments where researchers need to track gene expression over time, assess promoter activity, study cellular dynamics, or perform high-throughput screening.
Q: What advantages do Reporter Lentiviral Particles offer compared to other methods of introducing reporter genes?
A: Lentiviral particles efficiently transduce a broad range of cell types. Integration of reporter genes into the host genome ensures stable and heritable expression.
| Cat.No. | Product Name | Price |
|---|---|---|
| LV00926Z | CMV-GFP lentiviral particles | Inquiry |
| LV00928Z | LacZ lentiviral particles | Inquiry |
| LV00938Z | EF1A-GFP lentiviral particles | Inquiry |
| LV00949Z | RFP Lentivirus | Inquiry |
| LV00950Z | Citrine Lentivirus | Inquiry |
| LV00951Z | tdTOMATO Lentivirus | Inquiry |
| LV00952Z | mCherry Lentivirus | Inquiry |
| LV00958Z | CAG-GFP Lentivirus | Inquiry |
| LV00959Z | Synapsin-GFP Lentivirus | Inquiry |
| LV00962Z | EF1α-mCherry-Puro Lentivirus | Inquiry |
| LV00963Z | CAG-mCherry Lentivirus | Inquiry |
| LV00964Z | Synapsin-mCherry Lentivirus | Inquiry |
| LV00974Z | Synapsin-RFP Lentivirus | Inquiry |
| LV00975Z | HB9-GFP Lentivirus | Inquiry |
| LV00976Z | EF1α-tdTOMATO Lentivirus | Inquiry |
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