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A reporter gene is a gene that researchers attach to a regulatory sequence of another gene of interest in cell culture, animals or plants. Commonly used reporter genes that offer visually identifiable characteristics usually involve fluorescent (GFP) or luminescent (Luciferase) proteins. Reporter gene analysis provides a convenient, rapid, and sensitive assay that can be used to study gene delivery and gene expression. Reporter genes driven by different promoters allow accurate evaluation of promoter strength in vitro and in vivo.
Purified reporter lentiviruses are available as single reporter vectors with a fluorescent or bioluminescent marker or dual reporter vectors with the combination of fluorescent and bioluminescent markers. Choice of fluorescent markers include: eGFP, eYFP, eCFP, mCherry, mKate2, PLUM, RFP. Bioluminescent markers include Firefly, Renilla and Gaussia luciferase. These reporter lentiviruses provide a highly sensitive, large dynamic range and simple reporter method to measure the activities of a promoter, gene regulation studies in living mammalian cells and for functional genomics and drug screening cell signaling assays. Besides, the reporter lentivirus can also be used to generate pathway screening cell lines.
Over the years, scientists at Creative Biogene have developed the most comprehensive line of reporter lentiviruses for all your research applications. The lentiviruses express a luminescence or fluorescent reporter (firefly luciferase, renilla luciferase, RFP or GFP fluorescent marker) under a specific promoter or under a minimal promoter embedded with multiple tandem repeats with the pathway specific transcription factor binding motif. Our reporter lentiviruses are ready-to-use particles for safe and effective reporter gene delivery into a broad range of cell types, including primary cells and other difficult-to-transfect cell types. Select from our product list below to find the reporter lentivirus that’s right for you!
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