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microRNA mimics are double-stranded miRNA-like RNA that are designed to copy the functionality of mature endogenous miRNA upon transfection. Once these RNA fragments are introduced into cells, the miRNA mimics can bind specifically to the targeted gene. The result is posttranscriptional repression or more specifically translational inhibition of the gene. miR-mimics will act in a gene-specific fashion allowing for "miRNA-targeting" and "miRNA-gain-of-function" methods that are presently primarily used as exogenous tools to study gene function by targeting mRNA through miRNA-like actions in mammalian cells.
Creative Biogene can speed up your research by providing high-quality miRNA mimics. Our miRNA mimics are chemically synthesized double-stranded RNA oligonucleotides. These miRNA mimics are available at 5, 10 and 20 nmol guaranteed yield.
Please browse our miRNA Mimics collection to find your desired one. If you don't find the miRNA Mimics of your interest in our collection, we can also work for your project with the best price!
miRNAs are small noncoding RNA molecules that regulate gene expression by binding to messenger RNA (mRNA) transcripts, thereby inhibiting their translation into proteins. Dysregulation of miRNA expression has been implicated in many diseases, including cancer, cardiovascular disease, and neurodegenerative diseases. MicroRNA mimics are synthetic or modified RNA molecules. miRNA mimics can simulate the high-level expression of endogenous mature miRNA in cells to enhance the regulatory effect of endogenous miRNA. miRNA mimic is a powerful tool for studying the function of miRNA.
Applications for miRNA mimics include:
Case Study 1
This study identifies and validates the functional interaction between LINC00240 and miR-338-5p. miR-338-5p appears to function as a downstream target of negative regulation by LINC00240, and miR-338-5p can target METTL3 at the 3ʹ UTR to downregulate its expression. In gastric cancer (GC) tissues, the expression of miR-338-5p was negatively correlated with LINC00240, and the expression of miR-338-5p was negatively correlated with METTL3. Importantly, miR-338-5p inhibitor or METTL3 overexpression could rescue the inhibitory effects of LINC00240 knockdown on cell proliferation and migration and inhibit apoptosis induction in GC cells.
Figure 1. miR-338-5p mimic (Creative Biogene, Shirley, NY) marginally downregulated LINC00240 level in GC cells. (Wang G, et al, 2021)
Case Study 2
Researchers studied the role of leukemia stem cells in chemotherapy resistance and relapse in acute myeloid leukemia. MicroRNA-34a (miR-34a), which acts on a cell death regulatory pathway, was significantly downregulated in non-M3 acute myeloid leukemia stem cells compared with normal hematopoietic stem cells. Transfection with a miR-34a mimic triggers leukemia stem cell death and prevents leukemia. Bioinformatics analysis and dual-luciferase reporter gene assay showed that miR-34a targets the 3' untranslated region of histone deacetylase 2, and the reinforced expression of miR-34a remarkably stimulated the expression of histone deacetylase 2 in leukemia stem cells. Targeting leukemic stem cells to trigger cell death by upregulating miR-34a expression may be useful in the diagnosis and treatment of acute myeloid leukemia.
Figure 2. Transfection of miR-34a mimic affected the viability of AML cells. (MiR-34a mimic and negative control (NC) mimic, which served as the scramble control, were obtained from Creative Biogene) (Hu Y, et al. 2020)
Case Study 3
Researchers evaluated the function of miR-206 in hepatocellular carcinoma (HCC). The results showed that the expression of miR-206 was reduced in 27 cases of human liver cancer tissues compared with adjacent normal tissues. Abnormally increased expression of miR-206 in three HCC cell lines (SMMC-7721, HepG2, and Huh7) attenuated cell viability, migration, and invasion. Furthermore, researchers found that miR-206 targeted the 3'-UTR of the cMET gene for silencing, and restoration of cMET expression reversed the inhibitory effect of miR-206 on HCC. Tumor cells expressing miR-206 also showed delayed growth in in vivo experiments compared with controls.
Figure 3. Hoechst 33342 staining was carried out in each group of HepG2 cells expressing miR-206-mimic (Creative Biogene, Shirley, NY) or control cells. (Wang Y, et al. 2019)
Q: What is miRNA mimic?
A: miRNA mimics are synthetic or artificial molecules that are designed to mimic the function of endogenous microRNAs (miRNAs).
Q: What is the difference between miRNA mimic and inhibitor?
A: miRNA mimics are chemically synthesized miRNAs that mimic naturally occurring miRNAs when transfected into cells. miRNA inhibitors are single-stranded modified RNAs that specifically inhibit miRNA function after transfection.
Q: How does miRNA mimic work?
A: MiRNA mimics are chemically engineered double-stranded RNA molecules that have similar sequences to specific endogenous miRNAs. When introduced into cells, these mimics can bind to the target mRNA, effectively mimicking the action of the endogenous miRNA. This results in the downregulation of the target gene's expression.
Q: Which microRNAs are the predesigned mimics available for?
A: The predesigned mimics are available for all human, mouse, and rat microRNAs in the miRBase Database.
| Cat.No. | Product Name | Price |
|---|---|---|
| MIMH0010 | miR-hsa-miR-1 mimics | Inquiry |
| MIMH0011 | miR-hsa-miR-7-1-3p mimics | Inquiry |
| MIMH0012 | miR-hsa-miR-7-2-3p mimics | Inquiry |
| MIMH0013 | miR-hsa-miR-7-5p mimics | Inquiry |
| MIMH0014 | miR-hsa-miR-9-3p mimics | Inquiry |
| MIMH0015 | miR-hsa-miR-9-5p mimics | Inquiry |
| MIMH0016 | miR-hsa-miR-10b-3p mimics | Inquiry |
| MIMH0017 | miR-hsa-miR-10a-3p mimics | Inquiry |
| MIMH0018 | miR-hsa-miR-10b-5p mimics | Inquiry |
| MIMH0019 | miR-hsa-miR-10a-5p mimics | Inquiry |
| MIMH0020 | miR-hsa-miR-15a-3p mimics | Inquiry |
| MIMH0021 | miR-hsa-miR-15b-3p mimics | Inquiry |
| MIMH0022 | miR-hsa-miR-15a-5p mimics | Inquiry |
| MIMH0023 | miR-hsa-miR-15b-5p mimics | Inquiry |
| MIMH0024 | miR-hsa-miR-16-2-3p mimics | Inquiry |
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