circRNA Services

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Background

Circular RNAs (circRNAs) are characterized by covalently closed loop structures, non-linear splicing, and high stability. They can function as molecular sponges for microRNAs (miRNAs) or proteins and participate in transcriptional regulation, signal transduction, peptide translation, and biomarker formation. circRNA research is expanding across oncology, autoimmune diseases, neurodegenerative disorders, and metabolic dysregulation.

Figure 1. Schematic overview of circRNA biogenesis, major circularization mechanisms. (Chen L, et al., 2022)

Integrated circRNA Research Platform

Creative Biogene's circRNA research platform integrates high-throughput sequencing (RNA-Seq), high-density microarray analysis, functional validation assays (gain and loss-of-function), and circRNA–miRNA/RBP interaction studies, providing an end-to-end solution for basic research, translational medicine, drug target discovery, and biomarker identification.

Expression Analysis

Microarray analysis

Regulatory and Interaction Analysis

Analysis of circRNA expression regulation
Identification of target genes
Protein–circRNA interaction analysis
mRNA–circRNA interaction analysis

circRNA Screening and Identification

RNA-Seq
Bioinformatics analysis
Validation via FISH and qPCR
Northern blot confirmation of circular structure

Functional Studies

Loss-of-function analysis
Gain-of-function analysis
Mechanistic investigation of circRNA functions

The platform supports multiple species databases (human, mouse, rat, primates) and an automated data analysis system, enabling rapid processing from raw data to differential analysis, annotation, pathway enrichment, and interaction network construction.

Core Strengths and Research Assurance

Creative Biogene's platform directly addresses key challenges in circRNA studies—low abundance, algorithmic variability, high false-positive rates, and difficult validation—ensuring that every project delivers highly reliable, publication-ready, and experimentally verifiable results.

Industry-Leading circRNA-Specific QC System

circRNAs lack poly(A) tails, are extremely low in abundance, and share high sequence similarity with linear RNAs, making detection prone to tool bias and RNase R variability. To overcome these challenges, we developed a robust multi-layer QC system:

High-resolution RNA integrity profiling (RIN, DV200, degradation index)
Dual enrichment strategy with structure-dependent resistance correction
Library assessment including BSJ support, residual linear RNA, GC bias, and library saturation
Multi-algorithm integration (CIRI2, CirComPara2, Circtools) to generate consensus BSJ results
Cross-platform validation (qPCR, Sanger sequencing, RNase R titration curves)

Multidisciplinary Scientific Team for Strategic Project Design

Our team includes experts in RNA biology, circRNA biogenesis, splicing regulation, oncology, immunology, and computational biology, providing strategy-level guidance:

Rational selection of enrichment approaches, library types, and sequencing depth
Interpretation and reconciliation of multi-tool detection results
circRNA–miRNA–mRNA network analysis, identification of translational regulatory elements, and assessment of coding potential
Target prioritization and hypothesis-driven functional design
Support for grant applications, manuscript preparation, and scientific presentations

End-to-End Workflow with Flexible Modular Options

From sample to mechanistic insight, we provide a fully integrated circRNA workflow, with optional modular engagement:

RNase R / RiboMinus enrichment and full-length library construction
High-depth short-read sequencing with long-read validation
circRNA detection, annotation, quantification, differential expression, and WGCNA network analysis
Prediction of translation regulatory elements, open reading frames, and RBP binding sites
Functional validation (qPCR, Northern blot, Cas13/CRISPR KO, overexpression systems)

Case Study

In a study investigating translation initiation mechanisms in hematopoietic and epithelial cells, researchers transfected equal numbers of C57Bl6/J LKS and granulocyte–monocyte progenitor cells with 1 µg of EGFP-circRNA (Creative Biogene) using Lipofectamine MessengerMAXTM at a 1:2 ratio. Media was refreshed 12 hours post-transfection, and cells were analyzed 24 hours later, with transfection efficiency reaching approximately 10%. This experimental setup enabled the assessment of IRES/Cap-dependent and independent translation across differentiation states and under stress conditions.

Figure 2. IRES/Cap-mediated translation progressively increases during hematopoietic and hair follicle differentiation, as shown by bicistronic reporter analysis in mouse and human HSPCs, using circRNA-derived reporter constructs. (Mazzola MC, et al., 2025)

Applications

circRNA research has emerged as a critical frontier in life sciences. Creative Biogene's services apply to:

Exploring circRNA biogenesis and regulatory mechanisms.
Identifying tumor-specific circRNAs as potential diagnostic or prognostic biomarkers.
Investigating circRNA roles in T cell activation and tumor immune evasion.
Studying circRNA regulatory mechanisms in Alzheimer's and Parkinson's disease.
Identifying molecules associated with drug sensitivity via circRNA regulatory networks.
Designing stable RNA drug vectors and vaccine templates based on coding circRNAs.

Contact Us

Creative Biogene has established collaborations with multiple research institutions and biopharmaceutical companies, supporting mechanistic studies, candidate screening, and preclinical validation of circRNA-related projects. Regardless of the stage—differential expression profiling, functional mechanism elucidation, basic research, or clinical translation—Creative Biogene provides high-quality services and innovative technology to accelerate research outcomes.

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