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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SMMC-7721 is a human hepatocellular carcinoma (HCC) cell line established in 1977, derived from a 50-year-old male patient with primary liver cancer. The human liver cancer cell line SMMC-7721 expresses both EGFR and VEGFR, making it an indispensable tool in liver cancer research. These cells exhibit an epithelial-like morphology, characterized by rapid growth rates and stable biological properties under in vitro culture conditions. SMMC-7721 is frequently utilized to investigate the molecular mechanisms of hepatocarcinogenesis and tumor cell metabolism, as well as to evaluate the efficacy of novel anticancer agents—including traditional Chinese medicine extracts and targeted small-molecule drugs. It has become a standard model for studying the progression of hepatic malignancies and therapeutic interventions. |
| Tissue | Liver |
| Disease | Hepatocellular Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 50 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Hepatocellular carcinoma (HCC) pathogenesis and cell signaling research 2. Screening and toxicity testing of anti-tumor drugs 3. Study of tumor cell autophagy, apoptosis, and metabolic pathways 4. Investigation of liver cancer cell migration and invasion 5. Development of human tumor xenograft models in immunocompromised mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in nude mice |
| Karyotype | Aneuploid; hypertriploid |
| Genetic Profile | TP53 mutation; often used in studies of AFP (Alpha-Fetoprotein) expression |
| Phenotype | High metabolic activity; maintains features of malignant hepatocytes |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 3 to 8 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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